Influence of the Protective Antigen, Produced by Bacillus anthracis Asporogenic Recombinant Strain, on the Immune System of Laboratory Animals
https://doi.org/10.21055/0370-1069-2012-1(111)-84-87
Abstract
Assessment of immunogenicity and biological safety of protective antigen preparation obtained from asporogenic recombinant producer, B. anthracis 55ΔTPA-1(Spo-), is made on the mice (BALB/c species) and Guiney pigs models. It is shown that the development of adaptive immunity in the inoculated with purified protein laboratory animals is characterized by high titers of specific antibodies. Double immunization of biological models with antigen preparation confers protection from B. anthracis infection, commensurable with protective capacity of the live anthrax vaccine. Obtained are the data confirming the possibility to reduce the injected doze of the vaccine strain in case of subsequent single immunization with protective antigen. It is determined that recombinant protective antigen shows no sign of reactogenicity and does not damage thymocytes and splenocytes in laboratory animals when administered for immunization.
About the Authors
P. Yu. Popova
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
N. I. Mikshis
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
O. M. Kudryavtseva
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
A. Yu. Goncharova
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
L. V. Novikova
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
T. N. Kashtanova
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
Yu. A. Popov
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
E. A. Smol'kova
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
A. L. Kravtsov
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
T. N. Shchukovskaya
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
References
1. Marinin L.I., Onishchenko G.G., Kravchenko T.B., Dyatlov I.A., Tyurin E.A., Stepanov A.V. et al. [Human Anthrax: Epidemiology, Prophylaxis, Diagnostics, Treatment]. M.: ZAO MP “Gigiena”; 2008. 416 p.
2. Medical Laboratory Technologies. Reference Book. St. Petersburg; 2000.
3. Mikshis N.I., Kudryavtseva O.M., Shulepov D.V., Goncharova A.Yu., Bolotnikova M.F., Novikova L.V. et al. [Asporogenic recombinant producer of anthrax protective antigen]. Biotekhnologia. 2010; 4:25–33.
4. Onishchenko G.G., Vasil’ev N.T., Litusov N.V., Kharechko A.T., Vasil’ev P.G., Sadovoy N.V. et al. [Anthrax: Relevant Issues of Microbiology, Epidemiology, Clinical Picture, Treatment and Prophylaxis]. M.: Ministry of Health of the Russian Federation; 1999. 447 p.
5. Baillie L. Is new always better than old? The development of human vaccines for anthrax. Hum. Vaccin. 2009; 5(12):806-16.
6. Koehler T. Bacillus anthracis physiology and genetics. Mol. Aspects Med. 2009; 30:386-96.
7. Mock M., Fouet A. Anthrax. Microbiol. Rev. 2001; 55:647-71.
Review
For citations:
Popova P.Yu., Mikshis N.I., Kudryavtseva O.M., Goncharova A.Yu., Novikova L.V., Kashtanova T.N., Popov Yu.A., Smol'kova E.A., Kravtsov A.L., Shchukovskaya T.N. Influence of the Protective Antigen, Produced by Bacillus anthracis Asporogenic Recombinant Strain, on the Immune System of Laboratory Animals. Problems of Particularly Dangerous Infections. 2012;(1(111)):84-87. (In Russ.) https://doi.org/10.21055/0370-1069-2012-1(111)-84-87
Views:
794
Email this article 