Development of Methodological Approaches for Examination of Particularly Dangerous Infectious Diseases Agents by Means of Atomic Power Microscopy
https://doi.org/10.21055/0370-1069-2012-2(112)-62-64
Abstract
The atomic power microscopy (APM) is used to study the cell surface structure of particularly dangerous infectious diseases agents and to carry out the morphometric analysis. APM shows similar results with scanning electron microscopy. However, its application makes it possible to avoid time-consuming and labour-intensive procedures of samples preparing for testing by fixation, dehydration and sputtering of conducting layer. Methodological approach has been elaborated for preparing and analysis of samples of agents of particularly dangerous infectious diseases by means of APM. This approach includes a selection of optimal substrate, mode of disinfection and scanning of samples.
Keywords
616.91/98:578
About the Authors
D. V. Utkin
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
O. S. Kuznetsov
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
P. S. Erokhin
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
A. N. Spitsyn
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
O. A. Volokh
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
N. A. Osina
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
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Review
For citations:
Utkin D.V., Kuznetsov O.S., Erokhin P.S., Spitsyn A.N., Volokh O.A., Osina N.A. Development of Methodological Approaches for Examination of Particularly Dangerous Infectious Diseases Agents by Means of Atomic Power Microscopy. Problems of Particularly Dangerous Infections. 2012;(2(112)):62-64. (In Russ.) https://doi.org/10.21055/0370-1069-2012-2(112)-62-64
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