Diagnostic Nutrient Media for Isolation and Identification of Anthrax Agent
https://doi.org/10.21055/0370-1069-2012-2(112)-82-84
Abstract
Designed is a differential-diagnostic solid nutrient media for isolation and identification of anthrax agent. This media contains: nutrient base (enzymic hydrolysate of fishbone powder), agar-agar, D (+)-sorbite, pH indicator bromthymol blue, and broad-spectrum antibiotic (polymyxin). The media makes it possible to differentiate virulent (capsular) and avirulent (non-capsular) Bacillus anthracis strains, as well as closely related saprophyte microorganisms, depending upon the color and colony morphology.
Keywords
616.981.51:576.809.33
About the Authors
V. A. Govorunova
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
L. I. Marinin
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
R. I. Mironova
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
M. V. Khramov
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
A. N. Mokrievich
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
A. M. Baranov
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
References
1. Govorunova V.A., Matsarenko G.V., Marinin L.I., Stepanov A.V. [Solid nutrient media for identification of anthrax agent]. RF Patent 2125610.
2. Egorova I.Yu., Selyaninov Yu.O., Vologina I.V. [Comparative evalu- ation of methods of B. anthracis indication in soils]. Veterenariya. 2006; 6:26–30.
3. Marinin L.I., Dyatlov I.A., Mokrievich A.N., Bakhteeva I.V., Belova E.V., Borzilov A.I. et al. [Methods of Study of Anthrax Agent Biological Properties. Study Guide]. M.: Gigiena; 2009. 304 p.
4. Methodic Regulations [Laboratory diagnostics and detection of anthrax agent]. MR 4.2.2413-08. M.: Rospotrebnadzor Federal Center of Hygiene and Epidemiology; 2009. 67 p.
Review
For citations:
Govorunova V.A., Marinin L.I., Mironova R.I., Khramov M.V., Mokrievich A.N., Baranov A.M. Diagnostic Nutrient Media for Isolation and Identification of Anthrax Agent. Problems of Particularly Dangerous Infections. 2012;(2(112)):82-84. (In Russ.) https://doi.org/10.21055/0370-1069-2012-2(112)-82-84
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