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Studies of Biofilm Formation in Non-Pigmented and Plasmid-Deprived Mutants of Yersinia pestis on Biotic Surfaces, in vivo and in vitro Conditions

https://doi.org/10.21055/0370-1069-2012-3-45-49

Abstract

In non-pigmented and plasmid-deprived mutants – isogenic variants of highly virulent
Yersinia pestis 231 strain – studied is the mechanism of biofilm formation on biotic surfaces, both
in vitro (on the laboratory model of nematode
Caenorhabdiitis elegans) and
in vivo (inside the alimentary tract of
Nosopsyllus laeviceps flea). It is determined that spontaneous loss of ability to form biofilms and generate pigmented colonies in the mutants is probably caused not only by the deletion of the whole chromosome pigmentation fragment, but also by a point(single base) mutation in structural
hms operon. It is demonstrated that the absence of
pCad, pFra or
pPst plasmids does not have an impact on the ability of plasmid-deprived mutants to form biofilm on the cuticle of nematode
C. elegans.

About the Authors

G. A. Eroshenko
Russian Research Anti-Plague Institute “Microbe”
Russian Federation


N. A. Vidyaeva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation


L. M. Kukleva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation


E. I. Koshel’
Russian Research Anti-Plague Institute “Microbe”
Russian Federation


G. N. Odinokov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation


N. Yu. Shavina
Russian Research Anti-Plague Institute “Microbe”
Russian Federation


T. V. Knyazeva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation


T. V. Mokrousova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation


Ya. M. Krasnov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation


L. V. Anisimova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation


L. A. Novichkova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation


P. S. Erokhin
Russian Research Anti-Plague Institute “Microbe”
Russian Federation


A. V. Boiko
Russian Research Anti-Plague Institute “Microbe”
Russian Federation


V. V. Kutyrev
Russian Research Anti-Plague Institute “Microbe”
Russian Federation


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Review

For citations:


Eroshenko G.A., Vidyaeva N.A., Kukleva L.M., Koshel’ E.I., Odinokov G.N., Shavina N.Yu., Knyazeva T.V., Mokrousova T.V., Krasnov Ya.M., Anisimova L.V., Novichkova L.A., Erokhin P.S., Boiko A.V., Kutyrev V.V. Studies of Biofilm Formation in Non-Pigmented and Plasmid-Deprived Mutants of Yersinia pestis on Biotic Surfaces, in vivo and in vitro Conditions. Problems of Particularly Dangerous Infections. 2012;(3(113)):45-49. (In Russ.) https://doi.org/10.21055/0370-1069-2012-3-45-49

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ISSN 0370-1069 (Print)
ISSN 2658-719X (Online)