Comparative Study of Some Physical-Chemical and Immunochemical Properties of Plague Microbe Lipopolysaccharide Preparations Obtained with the Help of Different Techniques
https://doi.org/10.21055/0370-1069-2012-3-50-53
Abstract
, and degraded polysaccharide (PS) are easily soluble in water and in 0,9 % NaCl solution. They are homogenous and characterized by an adequate degree of purity. Aside from that, it is demonstrated that potentially PS is the most productive molecule fragment of LPS for the construction of plague immunodiagnostic preparation, since despite its decreased cytotoxocity PS retains identity of chemical composition and immunechemical specificity of endotoxin.
About the Authors
T. A. Polunina
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
N. P. Guseva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
M. N. Kireev
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
T. M. Taranenko
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
Z. L. Devdariani
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
S. P. Zadnova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
E. M. Kuznetsova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
A. V. Stepanov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
S. N. Klyueva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
T. L. Zakharova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
References
1. Bespalova I.A., Pustovalov V.L., L’vov V.L., Verner I.K., Vasil’eva G.I. [Method for acquisition and some properties of the modified Yersinia pes- tis lipopolysaccharide derivate]. Biotekhnologya. 1995; 9–10:3–4.
2. Dentovskaya S.V., Platonov M.E., Bakhteeva I.V., Anisimov A.P. [The presence of the complete lipopolysaccharide core structure is necessary for the activation of Yersinia pestis plasminogen]. Probl. Osobo Opasn. Infek. 2007; (93):49–51.
3. Dentovskaya S.V., Bakhteeva I.V., Titareva G.M., Shaikhutdinova R.Z., Kondakova A.N., Bystrova O.V. et al. [Structural diversity and endotoxin activity of Yersinia pestis lipopolysaccharide]. Biokhimiya. 2008; 73(2):37– 46.
4. Zyuzina V.P., Demidova G.V., Sokolova E.P., Bespalova I.A., Borodina T.N., Alekseeva L.P. [Comparative assay of the protein spectrum that can be found in lipopolysaccharide preparations of Yersinia pestis, cultivated at 28 and 37 °C]. Biotekhnologia. 2003; 3:20–4.
5. Egorov A.M., Osipov A.P., Dzantiev B.B., Gavrilova E.M. [Theory and Practice as Related to Enzyme-Linked Immunoassay]. M.; 1991. 288 p.
6. Novikov D.K., Novikova V.I. [Cell-Bond Techniques of Immunodiagnostics]. Minsk; 1979. P. 20–3.
7. Svarval’ A.V., Tseneva G.Ya., Shenderovich O.A. [Yersinia lipopoly- saccharide and its biological activity]. Zh. Mikrobiol. Epidemiol. Immunobiol. 2006; 3:100–4.
8. Sokolova E.P., Marchenkov V.I., Demidova G.V., Zyuzina V.P., Bespalova I.A., Pavlovich N.V. [Preparation complexes of plague microbe “mice” toxin with modified forms of Yersinia pestis lipopolysaccharide and lipopolysaccharides of other bacteria]. Biotekhnologya. 2001; 4:53–8.
9. Fedorova V.A., Devdariani Z.L. [Studies of Y. pestis lipopolysaccha- ride epitopes using monoclonal antibodies]. Mol. Genet. Mikrobiol. Virusol. 1998; 3:22–6.
10. Darveau R.P., Hancock R.T.W. Procedure for isolation of bacterial lipopolysaccharides from both smooth and rough Pseudomonas aeruginosa and Salmonella thyphimurium strains. J. Bacteriol. 1983; 155(2):831–8.
11. Galanos C., Luderitz O., Westphal O.A. New method for the extraction of R lipopolysaccharide. Eur. J. Biochem. 1969; 9:245–9.
12. Knirel Y.A., Lindner B., Vinogradov E.V., Kocharova N.A., Senchenkova S.N., Shaikhutdinova R.Z. et al. Temperature-dependent variations and intraspecies diversity of the structure of the lipopolysaccaride of Yersinia pestis. Biochemistry. 2005; 44:1731–43.
13. Muller-Seitz E., Jann B., Jann K. Degradation studies on the lipopolysaccharide from E. coli 071:K:H12. Separation and investigation of O-specific and core polysaccharides. FEBS Letters. 1968; 1(5):311–4.
14. Skurnik M., Peippo A., Ervela E. Characterization of the O-antigen gene clusters of Yersinia pseudotuberculosis and the cryptic O-antigen gene cluster of Yersinia pestis shows that the plague bacillus is most closely related to and has evolved from Y. pseudotuberculosis serotype O:1b. Mol. Microbiol. 2000; 37(2):316–30.
15. Westphal O., Luderitz O., Bister F. Uber die Extraktion von Bakterien mit Phenol. Wasser. Z. Naturforsch. Teil B. 1952; 7:148–55.
Review
For citations:
Polunina T.A., Guseva N.P., Kireev M.N., Taranenko T.M., Devdariani Z.L., Zadnova S.P., Kuznetsova E.M., Stepanov A.V., Klyueva S.N., Zakharova T.L. Comparative Study of Some Physical-Chemical and Immunochemical Properties of Plague Microbe Lipopolysaccharide Preparations Obtained with the Help of Different Techniques. Problems of Particularly Dangerous Infections. 2012;(3(113)):50-53. (In Russ.) https://doi.org/10.21055/0370-1069-2012-3-50-53
Views:
560
Email this article 