Impact of the Liquid Part and Some Separate Components of Blood on the Process of Interaction between 
                   Yersinia pestis  EV NIIEG Bacteria and Human Red Blood Cells 
                   in vitro

E. V. Pimenov; V. A. Oborin; A. G. Ivonin

doi:10.21055/0370-1069-2013-4-69-72

Impact of the Liquid Part and Some Separate Components of Blood on the Process of Interaction between Yersinia pestis EV NIIEG Bacteria and Human Red Blood Cells in vitro

E. V. Pimenov, V. A. Oborin, A. G. Ivonin

https://doi.org/10.21055/0370-1069-2013-4-69-72

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Abstract

Objective of the research has been the evaluation of impact of blood liquid part and its separate protein fractions on the process of Yersinia pestis EV NIIEG adhesion to human erythrocytes in vitro . Bacterial binding with target-cells has been registered using photometric technique. Identified has been inhibiting effect of blood native plasma and serum, as well as some groups of serum proteins, (albumins and normal immunoglobulins) on attachment of bacteria to human red blood cells. Based on the obtained results it has been assumed that there are two possible mechanisms of suppression of Yersinia pestis EV NIIEG adhesion to human erythrocytes performed by the liquid components of blood – surface microbial structure blockage and erythrocyte membrane receptor shielding.

Keywords

штамм Y pestis EV НИИЭГ, адгезивная активность, эритроциты, плазма и сыворотка крови, альбумины, нормальные иммуноглобулины, Yersinia pestis EV NIIEG strain, adhesive activity, erythrocytes, blood plasma and serum, albumin, common immunoglobulins

About the Authors

E. V. Pimenov

Komi Scientific Center of the Ural Branch of the Russian Academy of Sciences; Vyatka State Agricultural Academy
Russian Federation

V. A. Oborin

Vyatka State Humanities University
Russian Federation

A. G. Ivonin

Komi Scientific Center of the Ural Branch of the Russian Academy of Sciences; Vyatka State Agricultural Academy
Russian Federation

References

1. Anisimov A.P. [Yersinia pestis factors responsible for plague agent circulation and persistence in ecological systems of natural foci. Communication 2]. Mol. Gen. Mikrobiol. Immunol. 2002; 4:3–11.

2. Bibikova V.A., Klassovsky L.N. [Plague Transmission by Fleas]. M.: Meditsina; 1974. 188 p.

3. Vodop’yanov S.O., Mishan’kin B.N. [Pilli of adhesion in Yersinia pestis]. Zh. Mikrobiol. Epidemiol. Immunobiol. 1985; 6:13–7.

4. Dyatlov I.A., Antonova O.A. [Identification and characterization of Yersinia pestis antigen exerting S-layer protein properties]. Zh. Mikrobiol. Epidemiol. Immunobiol. 1999; 4:90–1.

5. Eropkina E.M., Afinogenov G.E. [Adhesive activity of protein complexes of biological liquids and secretions]. Zh. Mikrobiol. Epidemiol. Immunobiol. 1994; 11:110–6.

6. Kutyrev V.V. [Pressing issues of particularly dangerous infectious diseases and sanitary protection of the territories under current conditions]. Zh. Mikrobiol. Epidemiol. Immunobiol. 2008; 1:17–23.

7. Makarenkova I.D., Kompanets G.G., Zaporozhets T.C. [Inhibition of pathogenic microorganism adhesion onto eukaryotic cells]. Zh. Mikrobiol. Epidemiol. Immunobiol. 2006; 3:121–5.

8. Murav’ev A.V., Cheporov S.V. [Hemorheology (Experimental and Clinical Aspects)]. Yaroslavl: YSPU (Yaroslavl State Pedagogical University) Publishing House; 2009. 178 p.

9. Naumov A.V., Ledvanov M.Yu., Drozdov I.G. [Plague Immunology. Practice Guidance]. Saratov; 1992. 172 p.

10. Oborin V.A., Pimenov E.V., Ivonin A.G. [Analysis of adhesion of Yersinia pestis vaccine strain EV to erythrocytes of the animals using photocolorimetric method]. Probl. Osobo Opasn. Infek. 2010; 103:48–50.

11. Pimenov E.V., Oborin V.A., Ivonin A.G. [Investigation of mechanisms of interaction of Yersinia pestis EV NIIEG vaccine strain bacteria with human red blood cells]. Probl. Osobo Opasn. Infek. 2012; 114:54–7.

12. Stepanshina V.N., Gremyakova T.A., Anisimov A.P., Potapov V.D. [Physico-chemical and biological pH 6 characteristics of Yersinia pestis antigen isolated by means of immuno- adsorption technique]. Zh. Mikrobiol. Epidemiol. Immunobiol. 1996; 3:12–7.

13. Feodorova V.A., Devdariani Z.L. The interaction of Yersinia pestis with erythrocytes. J. Med. Microbiol. 2002; 51(2):150–8.

14. Mertz Р.М., Patti J.M., Marcin J.J., Marshall D.A. Model for studying bacterial adherence to skin wounds. J. Clin. Microbiol. 1987; 25(9):1601–4.

15. Perry R.D., Fetherston J.D. Yersinia pestis – etiologic agent of plague. Clin. Microbiol. Rev. 1997; 10:35–66.

Review

For citations:

Pimenov E.V., Oborin V.A., Ivonin A.G. Impact of the Liquid Part and Some Separate Components of Blood on the Process of Interaction between Yersinia pestis EV NIIEG Bacteria and Human Red Blood Cells in vitro. Problems of Particularly Dangerous Infections. 2013;(4):69-72. (In Russ.) https://doi.org/10.21055/0370-1069-2013-4-69-72

This work is licensed under a Creative Commons Attribution 4.0 License.

ISSN 0370-1069 (Print)
ISSN 2658-719X (Online)

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Problems of Particularly Dangerous Infections