Preview

Problems of Particularly Dangerous Infections

Advanced search

Phosphatase Activity in Chemical Cholera Vaccine and its Components

https://doi.org/10.21055/0370-1069-2012-3-58-60

Abstract

Presented are the data on detection and characterization of phosphomonoesterase and phosphodiesterase activities in detoxicated cultural fluid of production
Vibrio cholerae strains 569B and M41, and in choleragen-anatoxin and O-antigenic fraction, the vaccine components. These enzymes were demonstrated to be present in the cholera vaccine tablet, thus its biochemical properties were characterized more completely.

About the Authors

O. V. Gromova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation


I. A. Kuz’Michenko
Russian Research Anti-Plague Institute “Microbe”
Russian Federation


M. N. Kireev
Russian Research Anti-Plague Institute “Microbe”
Russian Federation


O. D. Klokova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation


V. S. Bronnikova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation


References

1. Dzhaparidze M.N., Nikitina G.P., Ivanov N.R., Rystsova E.A., Udalova I.B., Karaeva L.P. et al. [Biochemical and immunochemical char- acteristics of a new oral, chemical cholera bivalent vaccine and results of a trial of the preparation on volunteers]. Zh. Microbiol. Epidemiol. Immunobiol. 1982; 11:29–33.

2. Dzaparidze M.N., Naumov A.V., Nikitina G.P., Meleshchenko M.B. [An oral chemical vaccine from the hypertoxigenic strains of the causative agent of cholera KM-76 Inaba and KM-68 Ogawa]. Zh. Mikrobiol. Epidemiol. Immunobiol. 1991; 4:31–3.

3. Kuz’michenko I.A., Gromova O.V., Kireev M.N., Belyakova N.I. [Enzymatic activity in the dynamics of submerged cultivation of productional Vibrio cholerae strains O1 and O139 serogroups]. Probl. Osobo Opasn. Infek. 2003; (86):86–9.

4. Kuz’michenko I.A., Gromova O.V., Kireev M.N., Eremin S.A., Belyakova N.I., Klokova O.D. et al. [Monitoring of enzyme activities dur- ing production of cholera bivalent tablet vaccine]. Biotekhnologya. 2010; 2:87–92.

5. Metsler D. [Biochemistry: the chemical reactions of living cell]. M.; 1980.

6. Mishan’kin M.B., Vasil’eva G.I., Shimanyuk N.Ya., Shevchenko L.A., Kozlovsky V.N., Mishan’kin B.N. [Phosphatase activity of purified prepara- tions of Vibrio cholerae enterotoxin]. Probl. Osobo Opasn. Infek. 2003; (85):85–90.


Review

For citations:


Gromova O.V., Kuz’Michenko I.A., Kireev M.N., Klokova O.D., Bronnikova V.S. Phosphatase Activity in Chemical Cholera Vaccine and its Components. Problems of Particularly Dangerous Infections. 2012;(3(113)):58-60. (In Russ.) https://doi.org/10.21055/0370-1069-2012-3-58-60

Views: 485


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 0370-1069 (Print)
ISSN 2658-719X (Online)