Immunobiological Characteristics of Cholera Toxin B-Subunit
https://doi.org/10.21055/0370-1069-2012-3-67-70
Abstract
Carried out was immunobiological evaluation of cholera toxin B subunit preparations obtained using different experimental and production methods. It was demonstrated that B subunit preparations were non-toxic for biomodels and did not cause significant pathological alterations in their organs and tissues. They also did not alter the condition of immunocompetent cells at different stages of their cycle, and promote anti-toxic antibodies production. Thus, B subunit preparations under study can be considered as promising components of cholera vaccine.
About the Authors
S. N. Klyueva
Russian Research Anti-Plague Institute “Microbe”
Россия
Россия
T. N. Shchukovskaya
Russian Research Anti-Plague Institute “Microbe”
Россия
Россия
T. P. Shmel’Kova
Russian Research Anti-Plague Institute “Microbe”
Россия
Россия
A. L. Kravtsov
Russian Research Anti-Plague Institute “Microbe”
Россия
Россия
S. A. Bugorkova
Russian Research Anti-Plague Institute “Microbe”
Россия
Россия
N. I. Smirnova
Russian Research Anti-Plague Institute “Microbe”
Россия
Россия
O. A. Volokh
Russian Research Anti-Plague Institute “Microbe”
Россия
Россия
T. L. Zakharova
Russian Research Anti-Plague Institute “Microbe”
Россия
Россия
N. I. Belyakova
Russian Research Anti-Plague Institute “Microbe”
Россия
Россия
S. A. Eremin
Russian Research Anti-Plague Institute “Microbe”
Россия
Россия
A. K. Nikiforov
Russian Research Anti-Plague Institute “Microbe”
Россия
Россия
References
1. Ashmarin I.P., Vorob`ev A.A. [Statistical Methods in Microbiological Investigations]. L.; 1962. 180 p.
2. Voroshilova M.K., Zhevandrova V.I., Balayan M.S. [Methods of Laboratory Diagnostics of Enteroviral Infections]. M.; 1964. 152 p.
3. Zakharova T.L., Livanova L.F., Zadnova S.P., Smirnova N.I. [Obtaning of purified cholera toxin B-subunit from the recombinant Vibrio cholerae strain]. Biotechnologya. 2003; 5:53–6.
4. Il`ina T.S., Smirnov G.B., Smirnova N.I., Livanova L.F. [Recombinant plasmid pJEM3 DNA, encoding cholera toxin B-subunit, the method of its construction, and V. сholerae strain – producer of cholera toxin B-subunit]. AS 1505022.
5. Kutyrev V.V., Shchukovskaya T.N. [Cholera vaccines]. In: [Vaccines and Vaccination: National Manual]. M.; 2011. P. 431–45.
6. Lilly R. [Pathohystological Technique and Practical Hystochemistry]. M.; 1969. 645 p.
7. [Methods of Control of Immunobiological Preparations Administered to Humans]. MR. M.; 1998. 128 p.
8. Onishchenko G.G., Kutyrev V.V., Shchukovskaya T.N., Smirnova N.I., Nikiforov A.K. [Cholera specific prophylaxis in modern conditions]. Probl. Osobo. Opasn. Infek. 2011; (107):5–12.
9. Sarkisov D.S., Perov Yu.L. [Microscopic Technique: Manual]. M.; 1996. 542 p.
10. Shchukovskaya T.N., Sayapina L.V., Kutyrev V.V. [Cholera vaccino- prophylaxis: present day state]. Epidemiol. Vaktsynoprof. 2009; 2(45)
11. Barlogie B., Spidzer G., Hart G.S. et al. DNA histogram analysis of human hemopoetic cells. Blood. 1976; 48(2):245–57.
12. Chang Y.S., Sack D.A. Development of a novel in vitro assay (ALS assay) for evaluation of vaccine-induced antibody secretion from circulating mucosal lymphocytes. Clin. Diagn. Lab. Immunol. 2001; 8(3):482–8.
13. Lotzmanova E.Yu., Kravtzov A.L., Livanova L.F. et al. Flow cytofluorometric monitoring of leukocyte apoptosis in experimental cholera. Proc. SPIE. 2002; 5068:462–6.
14. Rhie G., Jung H., Park J. et al. Construction of cholera toxin B subunit-producing Vibrio cholerae strains using the Mariner-FRT transposon delivery system. FEMS Immunol. Med. Microbiol. 2008; 52:23–8.
Review
For citations:
Klyueva S.N., Shchukovskaya T.N., Shmel’Kova T.P., Kravtsov A.L., Bugorkova S.A., Smirnova N.I., Volokh O.A., Zakharova T.L., Belyakova N.I., Eremin S.A., Nikiforov A.K. Immunobiological Characteristics of Cholera Toxin B-Subunit. Problems of Particularly Dangerous Infections. 2012;(3(113)):67-70. (In Russ.) https://doi.org/10.21055/0370-1069-2012-3-67-70
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