Algorithm of Laboratory Diagnostics Applied for Examination of Ixodic Ticks for Tularemia
https://doi.org/10.21055/0370-1069-2012-3-79-81
Abstract
Put forward is the algorithm of laboratory diagnostics applied for examination of Ixodic ticks (IT) for tularemia. It involves carrying out primary screening of tick suspensions for the presence of species-specific fragments of DNA and antigen. It is recommended that laboratory analysis starts with PCR, taking into account its high sensitivity and diagnostic value. Pools with positive PCR tests are subjected to verification by means of enzyme-linked immunosorbent assay and immune-chromatographic tests. In case of tularemia antigen detection, these pools of IT are examined with the help of biological or bacteriological techniques to isolate the agent.
About the Authors
A. A. Zaitsev
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
O. A. Gnusareva
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
B. V. Solodovnikov
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
N. S. Tsareva
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
V. V. Ostapovich
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
A. N. Kulichenko
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
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Review
For citations:
Zaitsev A.A., Gnusareva O.A., Solodovnikov B.V., Tsareva N.S., Ostapovich V.V., Kulichenko A.N. Algorithm of Laboratory Diagnostics Applied for Examination of Ixodic Ticks for Tularemia. Problems of Particularly Dangerous Infections. 2012;(3(113)):79-81. (In Russ.) https://doi.org/10.21055/0370-1069-2012-3-79-81
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