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Comparative Analysis of Immunochemical Methods Applied for Studies of Pathogenic Burkholderia Antigens

https://doi.org/10.21055/0370-1069-2012-3-82-85

Abstract

Studied are immunochemical properties of antigen preparations, the spectrum and molar masses of the components contained. Demonstrated is the significance of vertical SDS-polyacrylamide gel electrophoresis, immunodiffusion test, immunoelectrophoresis assay, rocket immunoelectrophoresis with specific sera for identification and differentiation of
Burkholderia. Rocket immunoelectrophoresis should be viewed as the most informative method which allows for differentiation between pathogenic and non-pathogenic for humans
Burkholderia under usual terms.

About the Authors

I. I. Korsakova
Volgograd Research Anti-Plague Institute
Russian Federation


N. P. Khrapova
Volgograd Research Anti-Plague Institute
Russian Federation


G. M. Napalkova
Volgograd Research Anti-Plague Institute
Russian Federation


L. V. Lomova
Volgograd Research Anti-Plague Institute
Russian Federation


N. M. Drefs
Volgograd Research Anti-Plague Institute
Russian Federation


Yu. A. Goloseev
Volgograd Research Anti-Plague Institute
Russian Federation


T. V. Bulatova
Volgograd Research Anti-Plague Institute
Russian Federation


References

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Review

For citations:


Korsakova I.I., Khrapova N.P., Napalkova G.M., Lomova L.V., Drefs N.M., Goloseev Yu.A., Bulatova T.V. Comparative Analysis of Immunochemical Methods Applied for Studies of Pathogenic Burkholderia Antigens. Problems of Particularly Dangerous Infections. 2012;(3(113)):82-85. (In Russ.) https://doi.org/10.21055/0370-1069-2012-3-82-85

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ISSN 0370-1069 (Print)
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