Application of Basophil Activation Test with Anthraxin for Laboratory ( in vitro) Diagnostics of Anthrax
https://doi.org/10.21055/0370-1069-2012-3-86-88
Abstract
Demonstrated is the possibility to use
in vitro basophil activation test with anthraxin, with registration of the results by means of flow cytometry, for anthrax diagnostics. This approach seems promising as it provides quantitative assessment of sensitization of the organism and does not cause its additional allergization. The duration of the analysis is 1h. The test is suggested for application, as an express one, for early and retrospective laboratory diagnostics of anthrax, estimation of post-vaccinal immunity.
in vitro basophil activation test with anthraxin, with registration of the results by means of flow cytometry, for anthrax diagnostics. This approach seems promising as it provides quantitative assessment of sensitization of the organism and does not cause its additional allergization. The duration of the analysis is 1h. The test is suggested for application, as an express one, for early and retrospective laboratory diagnostics of anthrax, estimation of post-vaccinal immunity.
About the Authors
A. N. Kulichenko
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
E. L. Rakitina
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
D. G. Ponomarenko
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
O. V. Logvinenko
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
A. G. Ryazanova
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
References
1. Bakulov I.A., Gavrilov V.A., Seliverstov V.V. [Anthrax. New pages in histories of old disease].Vladimir; 2001. 278 p.
2. Novikov D.K., Sergeev Yu.V., Novikov P.D. [Drug allergy]. M: 2001. 330 p.
3. Onishchenko G.G., Vasil’ev N.T., Litusov N.V., Kharchenko A.T., Vasil’ev P.G., Sadovoi I.V., Kozhukhova V.V. [Anthrax: actual aspects of micro- biology, epidemiology, clinic, diagnostics and prophylaxis]. M.; 1999. 448 p.
4. Onishchenko G.G., Kozhukhov V.V. [Anthrax]. M.; 2010. 424 p. 5. Shlyakhov E.N. [Anthrax. Biological and immunological principles of diagnostics and prevention.
5. The dynamics and intensity of skin tests with anthraxin in guinea pigs inoculated with a STI vaccine]. Zh. Hyg. Epidemiol. Microbiol. Immunol. 1970; 3:364–8.
6. Shchukovskaya T.I., Firstova V.V., Kravtsov A.L., Klyueva S.N., Popov Yu.A., Mikshis N.I. [Estimation of the acquired immunity against an- thrax according to the extent of blood leucocytes damage in vitro caused by anthraxin]. Probl. Osobo Opasn. Infek. 2007; (93):81–4.
7. De Weck A.L., Sanz M.L. Flow cytometric cellular allergen stimulation test. Technical and clinical evaluation of a new diagnostic test in allergy and pseudo–allergy. ACI International. 2002; 4(5):204–15.
8. Shlyakhov E. Anthraxin-α skin test for early and retrospective diagnosis of anthrax and anthrax vaccination assessment. Salisbury Med Bull Suppl. 1996; 87:109–10.
Review
For citations:
Kulichenko A.N., Rakitina E.L., Ponomarenko D.G., Logvinenko O.V., Ryazanova A.G. Application of Basophil Activation Test with Anthraxin for Laboratory ( in vitro) Diagnostics of Anthrax. Problems of Particularly Dangerous Infections. 2012;(3(113)):86-88. (In Russ.) https://doi.org/10.21055/0370-1069-2012-3-86-88
Views:
685
Email this article 