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Problems of Particularly Dangerous Infections

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Construction of nutrient media for L-form brucella

https://doi.org/10.21055/0370-1069-2012-3-89-93

Abstract

Dry nutrient media on the basis of hepatic infusion and Siberian roach hydrolyzate are constructed. Experimental data demonstrate that these media possess high sensitivity, completely inhibit growth of S-form
Brucella, do not require рН adjustment, filtration and autoclaving. They can be used for isolation, cultivation and accumulation of L-form
Brucella for bacteriological diagnostics of brucellosis. As these media are accessible for transportation, they are applicable for stationary and field conditions.

About the Authors

L. M. Mikhailov
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Russian Federation


A. I. Kalinovsky
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Russian Federation


N. L. Barannikova
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Russian Federation


V. I. Kuznetsov
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Russian Federation


A. G. Atlas
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Russian Federation


N. M. Andreevskaya
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Russian Federation


V. A. Mikhailova
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Russian Federation


O. G. Tatarnikova
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Russian Federation


L. N. Gordienko
Russian Research Institute of Animal Brucellosis and Tuberculosis of RAAS
Russian Federation


E. V. Kulikova
Russian Research Institute of Animal Brucellosis and Tuberculosis of RAAS
Russian Federation


S. V. Balakhonov
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Russian Federation


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Review

For citations:


Mikhailov L.M., Kalinovsky A.I., Barannikova N.L., Kuznetsov V.I., Atlas A.G., Andreevskaya N.M., Mikhailova V.A., Tatarnikova O.G., Gordienko L.N., Kulikova E.V., Balakhonov S.V. Construction of nutrient media for L-form brucella. Problems of Particularly Dangerous Infections. 2012;(3(113)):89-93. (In Russ.) https://doi.org/10.21055/0370-1069-2012-3-89-93

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ISSN 0370-1069 (Print)
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