Construction of nutrient media for L-form brucella
https://doi.org/10.21055/0370-1069-2012-3-89-93
Abstract
Dry nutrient media on the basis of hepatic infusion and Siberian roach hydrolyzate are constructed. Experimental data demonstrate that these media possess high sensitivity, completely inhibit growth of S-form
Brucella, do not require рН adjustment, filtration and autoclaving. They can be used for isolation, cultivation and accumulation of L-form
Brucella for bacteriological diagnostics of brucellosis. As these media are accessible for transportation, they are applicable for stationary and field conditions.
Brucella, do not require рН adjustment, filtration and autoclaving. They can be used for isolation, cultivation and accumulation of L-form
Brucella for bacteriological diagnostics of brucellosis. As these media are accessible for transportation, they are applicable for stationary and field conditions.
About the Authors
L. M. Mikhailov
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Россия
Россия
A. I. Kalinovsky
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Россия
Россия
N. L. Barannikova
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Россия
Россия
V. I. Kuznetsov
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Россия
Россия
A. G. Atlas
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Россия
Россия
N. M. Andreevskaya
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Россия
Россия
V. A. Mikhailova
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Россия
Россия
O. G. Tatarnikova
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Россия
Россия
L. N. Gordienko
Russian Research Institute of Animal Brucellosis and Tuberculosis of RAAS
Россия
Россия
E. V. Kulikova
Russian Research Institute of Animal Brucellosis and Tuberculosis of RAAS
Россия
Россия
S. V. Balakhonov
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Россия
Россия
References
1. [Safety of work with microorganisms of I–II pathogenicity (danger) groups. SR 1.3.1285-03]. M.; 2003. 82 p.
2. Zykin L.F., Vasil’ev D.A. [L-Forms of Zoonoses Agents]. Ul`ya¬ novsk; 2000. 68 p.
3. Kosilov I.A., Arakelyan P.K., Dimov S.K. et al. [Brucellosis of Agri¬ cultural Animals]. Novosibirsk. 1999. 343 p.
4. Mikhailov L.M., Maevsky M.P., Kuznetsov V.I., Kalinovsky A.I., Tatarnikova O.G. [Nutrient media for L-form Brucella isolation and cultivation]. RF Patent 2263142.
5. Mikhailov L.M., Kalinovsky A.I., Andreevskaya N.M., Golubinsky E.P., Mikhailova V.A., Repina L.P. et al. [Method for obtaining Brucella abor- tus strain I-206 L-subcultures]. RF Patent 2263142.
6. Mikhailov L.M., Kalinovsky A.I., Repina L.P. et al. [Epidemiological and epizootiological significance of L-form Brucella]. Bul. RAMS. 2002; 3:49–53.
7. Oshchepkov V.G., Kopeikin I.G., Stepanov E.M. et al. [L-trans¬ formation of B. ovis on artificial nutrient media in the organism of sheep]. In: [Diagnostics and Prophylaxis of Infectious and Invasive Diseases of Agricultural Animals]. Omsk; 1986. P. 21–8.
8. [Infectious diseases prophylaxis. Infections common for both hu- mans and animals. Brucellosis prophylaxis and bacteriological diagnostics. MR 3.1.7.1189-03]. M.; 2003. 38 p.
9. Tolmacheva T.A., Kats L.N. [Biological properties and ultrastructure of brucella in the process of L-transformation and reversion]. Zh. Mikrobiol. Epidemiol. Immunobiol. 1977; 1:90–3.
10. Trilenko P.A. [Brucellosis of Agricultural Animals]. L.; 1976. 279.
11. Nelson E.L., Pickett M.J. The covery of Brucella and their relation to Brucella phage. J. Infect. Disease. 1951; 8(3):226–32.
Review
For citations:
Mikhailov L.M., Kalinovsky A.I., Barannikova N.L., Kuznetsov V.I., Atlas A.G., Andreevskaya N.M., Mikhailova V.A., Tatarnikova O.G., Gordienko L.N., Kulikova E.V., Balakhonov S.V. Construction of nutrient media for L-form brucella. Problems of Particularly Dangerous Infections. 2012;(3(113)):89-93. (In Russ.) https://doi.org/10.21055/0370-1069-2012-3-89-93
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