Qualitative Indicators of Microbial Cells of Yersinia pestis EV Strain Depending on Their Morphological Traits in Different Temperature Conditions of Manufacturing of Plague Vaccine Preparation
https://doi.org/10.21055/0370-1069-2012-4-33-35
Abstract
The preference of the (21±1) °C temperature for cultivation of the vaccine strain Yersinia pestis EV in the live plague vaccine biomass production to (27±1) °C is experimentally confirmed. An original interpretation of the mechanism of small cells stability that are formed at (21±1) °C has been proposed. This interpretation stipulates a smaller damaging effect of steam molecules during sublimation in the process of lyophilization.
About the Authors
D. A. Budyka
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
A. I. Bondarenko
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
A. A. Fisun
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
N. V. Abzaeva
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
G. F. Ivanova
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
S. E. Gostishcheva
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
S. M. Rudnev
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
References
1. Alutin I.M., Domaradsky I.V., Suichmezova A.V. [Study of the propa- gation dynamics in some species of microorganisms using polythermostat]. Probl. Osobo Opasn. Infek. 1973; 29:182–9.
2. Blankov B.I., Klebanov D.A. [ Application of the Lyophilization in Microbiology]. M.: Medgiz; 1961. P. 209–11.
3. Budyka D.A., Abzaeva N.V., Ivanova G.F., Fisun A.A., Lyapustina L.V. [Comparative analysis of experimental series of plague live vaccine as for viability and thermostability indicies]. Probl. Osobo Opasn. Infek. 2009; 102:68–71.
4. Eremenko Yu.D., Byvalov A.A., Pimenov E.V. [Peculiarities of fatty-acid composition of Yersinia pseudotuberculosis cells grown on solid nutritional medium at different temperatures]. Probl. Osobo Opasn. Infek. 2005; 89:41–3
5. Oivin I.A. [Statistical prosessing of results from experimental inves- tigations]. Patol. Phisiol. 1960; 4:76–85.
Review
For citations:
Budyka D.A., Bondarenko A.I., Fisun A.A., Abzaeva N.V., Ivanova G.F., Gostishcheva S.E., Rudnev S.M. Qualitative Indicators of Microbial Cells of Yersinia pestis EV Strain Depending on Their Morphological Traits in Different Temperature Conditions of Manufacturing of Plague Vaccine Preparation. Problems of Particularly Dangerous Infections. 2012;(4(114)):33-35. (In Russ.) https://doi.org/10.21055/0370-1069-2012-4-33-35
Views:
520
Email this article 