Phenotypic and Molecular-Genetic Analysis of Genetically Modified Toxigenic Vibrio cholerae El Tor Strain 301, Isolated in 2011 in Russia
https://doi.org/10.21055/0370-1069-2012-4-61-64
Abstract
The phenotypic and molecular-genetic analysis of
Vibrio cholerae O1 El Tor Inaba strain 301, isolated in 2011 from sea water in recreation zone in Taganrog, demonstrated this isolate to be a genetic variant of El Tor cholera causative agent. Its genome was shown to carry a hybrid prophage, containing gene ctxB of classical type and gene rstR of El Tor type, as well as altered pathogenicity island VPI-1 and pandemicity island VSP-II. This strain produced much more cholera toxin (0,12 mcg/ml) than typical strains of this causative agent.
Vibrio cholerae O1 El Tor Inaba strain 301, isolated in 2011 from sea water in recreation zone in Taganrog, demonstrated this isolate to be a genetic variant of El Tor cholera causative agent. Its genome was shown to carry a hybrid prophage, containing gene ctxB of classical type and gene rstR of El Tor type, as well as altered pathogenicity island VPI-1 and pandemicity island VSP-II. This strain produced much more cholera toxin (0,12 mcg/ml) than typical strains of this causative agent.
About the Authors
A. V. Shashkova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
D. A. Agafonov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
A. V. Cherkasov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
S. P. Zadnova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
N. I. Smirnova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
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Review
For citations:
Shashkova A.V., Agafonov D.A., Cherkasov A.V., Zadnova S.P., Smirnova N.I. Phenotypic and Molecular-Genetic Analysis of Genetically Modified Toxigenic Vibrio cholerae El Tor Strain 301, Isolated in 2011 in Russia. Problems of Particularly Dangerous Infections. 2012;(4(114)):61-64. (In Russ.) https://doi.org/10.21055/0370-1069-2012-4-61-64
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