Evaluation of Immunobiological Properties of Cholera Vibrio O1 and O139 Antigens
https://doi.org/10.21055/0370-1069-2012-4-77-79
Abstract
Evaluated are immunobiological properties of experimental preparation on the basis of
Vibrio cholerae O-antigen, O1 and O139 serogroups on the mouse model: determination of toxicity, analysis of morphological changes in organs, and flow-cytometric monitoring of cell cycle of leukocytes, splenocytes and bone marrow cells. It is demonstrated that O-antigen 100 µg dose per mouse does not initiate either significant changes in parenchymatous organs, or imbalance of apoptosis and proliferation of immune-competent cells. Comprehensive assessment of immunobiological properties of the preparations allows to get an insight about their effect on macroorganism, possibility for further studies and their application as chemical vaccine components.
Vibrio cholerae O-antigen, O1 and O139 serogroups on the mouse model: determination of toxicity, analysis of morphological changes in organs, and flow-cytometric monitoring of cell cycle of leukocytes, splenocytes and bone marrow cells. It is demonstrated that O-antigen 100 µg dose per mouse does not initiate either significant changes in parenchymatous organs, or imbalance of apoptosis and proliferation of immune-competent cells. Comprehensive assessment of immunobiological properties of the preparations allows to get an insight about their effect on macroorganism, possibility for further studies and their application as chemical vaccine components.
About the Authors
T. P. Shmel’Kova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
S. N. Klyueva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
S. A. Bugorkova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
A. L. Kravtsov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
O. V. Gromova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
O. A. Volokh
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
S. A. Eremin
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
A. K. Nikiforov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
T. N. Shchukovskaya
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
References
1. Zakharova T.L., Zadnova S.P., Livanova L.F., Kireev M.N., Smirnova N.I. [Recombinant strains application for simultaneous prepara- tion of several purified cholera vibrio antigens]. Probl. Osobo Opasn. Infek. 2009; (100):68–71.
2. Kutyrev V.V., Shchukovskaya T.N. [Cholera vaccines]. In: [Vaccine and Vaccination: National Guidelines]. M.; 2011. P. 431–45.
3. Lomov Yu.M., Moskvitina E.A. [Epidemiologic situation on chol- era in the world, CIS countries and Russia. Prognosis]. Probl. Osobo Opasn. Infek. 2010; (104):11–3.
4. [Methods of quality control over immunobiological preparations ad- ministered to humans. MR 4.1/4.2.588-96]. M.; 1996.
5. Sarkisova D.S., Perova Yu.L., editors. [Microscopy Equipment: Operating Manual]. M.: Meditsina; 1996. 544 p.
6. Shakhlamov V.A., Solnyshkova T.G. [Ultra-structural and morpho- logical analysis of Paneth cell reaction to cholera toxin administration]. Byul. Eksp. Biol. Med. 1992; 63(4):415.
7. Shchukovskaya T.N., Sayapina L.V., Kutyrev V.V. [Vaccine prophy- laxis of cholera: current state of the issue]. Epidemiol. Vaktsinoprofilakt. 2009; 2(45):62–7.
8. Barlogie B., Spidzer G., Hart G. et al. DNA histogram analysis of human hemopoetic cells. Blood. 1976; 48(2):245–57.
9. Final Report of the Independent Panel of Experts on the Cholera Outbreak in Haiti. 04 May 2011. Available from: http://www.un.org/News/dh/infocus/haiti/UN-cholera-report-final
10. Lotzmanova E.Yu., Kravtzov A.L., Livanova L.F. et al. Flow cytofluorometric monitoring of leukocyte apoptosis in experimental cholera. Proc. SPIE. 2002; 5068:462–6.
Review
For citations:
Shmel’Kova T.P., Klyueva S.N., Bugorkova S.A., Kravtsov A.L., Gromova O.V., Volokh O.A., Eremin S.A., Nikiforov A.K., Shchukovskaya T.N. Evaluation of Immunobiological Properties of Cholera Vibrio O1 and O139 Antigens. Problems of Particularly Dangerous Infections. 2012;(4(114)):77-79. (In Russ.) https://doi.org/10.21055/0370-1069-2012-4-77-79
Views:
487
Email this article 