Detection of Clones with Different Expression of Virulence and Persistence Factors in Natural Populations of Cholera Vibrio and Their Phenotypic Analysis
https://doi.org/10.21055/0370-1069-2009-3(101)-39-43
Abstract
Analysis of the population structure of 41 Vibrio cholerae biovar eltor natural strains permitted to detect 12 strains forming typical transparent and atypical opaque colonies. Among the later were identified 3 strains which morphology variability correlated with altered production of exopolysaccharide, cholera toxin, hemolysine, soluble hemagglutinin/protease and altered mobility. One of the external signals causing coordinating switching of production of the indicated pathogenicity and persistence factors is alkaline (pH 9,0) reaction of the medium.
Keywords
Vibrio cholerae,
heterogeneous population,
coordinated change of virulence and persistence gene expression
616.932:576.8.097.21
About the Authors
S. P. Zadnova
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
L. F. Livanova
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
Yu. V. Lozovskiy
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
N. I. Smirnova
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
References
1. Адамов А.К., Наумшина М.С. Холерные вибрионы. Саратов: Изд-во Сарат. ун-та; 1984. 328 с.
2. Исаев Н.Д., Лозовский Ю.В., Заднова С.П., Смирнова Н.И. Популяционная неоднородность природных штаммов Vibrio cholerae классического биовара: координированное изменение морфологии колоний, подвижности, токсигенности и ферментативных свойств. Пробл. особо опасных инф. 2005; 1(89):43-6.
3. Милютин В.Н., Дрожевкина М.С., Ломов Ю.М., Уралева В.С., Либинзон А. Е., Подосинникова Л.С. Механизмы и диапазон изменчивости холерных вибрионов. Ростов н/Д: Ростовское книжное изд-во; 1981. 176 с.
4. De S.N., Chatterje D.N. An experimental study of the mechanism of action of Vibrio cholerae on the intestinal mucous membrane. J. Path. Bacteriol. 1953; 66(2):559-62.
5. DiRita V.J., Neely M., Taylor R.K., Bruss P.M. Differential expression of the ToxR regulon in classical and El Tor biotypes of Vibrio cholerae is due to biotype-specific control over toxT expression. Proc. Natl. Acad. Sci. USA. 1996; 93(15):7991-95.
6. Faruque S.M., Nair G.B. Vibrio cholerae Genomics and Molecular Biology. Caister Academic Press, Norfolk, UK; 2008. 218 p.
7. Finkelstein R.A., Boesman-Finkelstein M., Chang Y., Hдse C.C. Vibrio cholerae hemagglutinin/protease, colonial variation, virulence and detachment. Infect. Immun. 1992; 60:472-8.
8. Guvener Z.T., McCarter L.L. Multiple regulators control capsular polysaccharide production in Vibrio parahaemolyticus. J. Bacteriol. 2003; 185(18):5431-41.
9. Hammer B.K., Bassler B.L. Distinct sensory pathways in Vibrio cholerae El Tor and classical biotypes modulate cyclic dimeric GMP levels to control biofilm formation. J. Bacteriol. 2009; 191(1):169-77.
10. Hilton T., Rosche T., Froelich B., Smith B., Oliver J. Capsular polysaccharide phase variation in Vibrio vulnificus. Appl. Environ. Microbiol. 2006; 72(11):6986-93.
11. Hitchсock P.J., Brown T.M. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J. Bacteriol. 1983; 154:269-77.
12. Iwanaga M., Yamamoto K., Higa N., Ichinose Y., Nakasone N., Tanabe M. Culture conditions for stimulating cholera toxin production by Vibrio cholerae O1 El Tor. Microbiol. Immunol. 1986; 30:1075-83.
13. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T. Nature. 1970; 227:680-5.
14. Nesper J., Lauriano C.M., Klose K.E., Kapfhammer D., Kraiβ A., Reidl J. Characterization of Vibrio cholerae O1 El Tor galU and galE mutants: influence on lipopolysaccharide structure, colonization, and biofilm formation. Infect. Immun. 2001; 69(1):435-45.
15. Svennerholm A.M., Wiklund G. Rapid GM1-enzyme-linked immunosorbent assay with visual reading for identification of Escherichia coli heat-labile enterotoxin. J. Clin. Microbiol. 1983; 17:596-600.
16. Yildiz F.H., Lie X.S., Heydorn A., Schoolnik G.K. Molecular analysis of rugosity in a Vibrio cholerae O1 El Tor phase variant. Mol. Microbiol. 2004; 53(2):497-515.
Review
For citations:
Zadnova S.P., Livanova L.F., Lozovskiy Yu.V., Smirnova N.I. Detection of Clones with Different Expression of Virulence and Persistence Factors in Natural Populations of Cholera Vibrio and Their Phenotypic Analysis. Problems of Particularly Dangerous Infections. 2009;(3(101)):39-43. (In Russ.) https://doi.org/10.21055/0370-1069-2009-3(101)-39-43
Views:
433
Email this article 