Production of S-layer proteins by Different Bacillus anthracis Strains
https://doi.org/10.21055/0370-1069-2008-4(98)-29-32
Abstract
Investigated were antigenic properties of proteins produced by B. anthracis strains STI (рХО1+, рХО2-), Davies (рХО1-, рХО2+), 81/1TR (рХО1-, рХО2-), eluated in void volume (fraction 1) at division of cultural filtrates (CF) on superfine sefacryle S-300. Sera to fractions 1 of B. anthracis STI, 81/1TR and Davies CF were shown to contain antibodies to different antigens. The proteins identified with the help of sera were referred to antigens of В. anthracis S-layer relaying on м.м. 94 kDa, characteristic for S-layer proteins, localization on superficial cell structure, and independence of their production from virulence plasmids.
About the Authors
I. A. Barkova
Volgograd Research Anti-Plague Institute
Russian Federation
Russian Federation
V. V. Alexeev
Volgograd Research Anti-Plague Institute
Russian Federation
Russian Federation
A. V. Lipnitsky
Volgograd Research Anti-Plague Institute
Russian Federation
Russian Federation
A. M. Barkov
Volgograd Research Anti-Plague Institute
Russian Federation
Russian Federation
L. V. Bukhantsova
Volgograd Research Anti-Plague Institute
Russian Federation
Russian Federation
References
1. Баркова И.А., Липницкий А.В., Барков А.М., Евтеева Е.В. Использование иммуноглобулинов к отдельным внеклеточным антигенам Bacillus anthracis СТИ для идентификации сибиреязвенного микроба. Биотехнология. 2005; 2:91-5.
2. Ермакова И.А., Липницкий А.В., Барков А.М., Евтеева Е.В. Способ выделения высокомолекулярного соматического сибиреязвенного антигена. Патент на изобретение № 2230570 от 20.06.2004 г.
3. Микшис Н.И., Корсакова А.Ю., Болотникова М.Ф. и др. Продукция белков S-слоя штаммами Bacillus anthracis. Биотехнология. 2004; 5:22-3.
4. Носков А.Н., Кравченко Т.Б., Носкова В.П. Выявление функционально-активных доменов в молекулах протективного антигена и летального фактора сибиреязвенного экзотоксина. Вестник РАМН. 1997; 6:20-4.
5. Шаханина К.Л. Приготовление люминисцирующих антител. Иммунология в медицине. Под ред. Левиной Е.Н. М.: Медицина; 1977. 237 с.
6. Chitlaru Т., Gat O. Differential proteomic analysis of the Bacillus anthracis secretome: distinct and chromosome CO2-dependent cross talk mechanisms modulate extracellular proteolytic activates. J. Bacteriol. 2006; 188(10):3551-71.
7. Ezzel J., Abshire T. Immunological analysis of cell associated antigens of Bacillus anthracis. Infect. Jmmun. 1988; 56:349-56.
8. Green B.D., Laurie Battisti, Kochler Т.М. et al. Demonstration of capsule in B. anthracis. Infect. Immun. 1985; 49(2):291-7.
9. Lamonica J.M., Wagner M.A., Echenbrenner M. Comparative secretome analyses of the Bacillus anthracis strains with variant plasmid contents. Infect. Immun. 2005; 73(6):3646-58.
10. Mignot T., Mesnage S., Counture-Tosi E. et al. Developmental switch of S-layer protein syntesis in Bacillus anthracis. Mol. Microbiol. 2002; 43:1615-28.
11. Mignot T., Mock M., Fouet A. A plasmid-encoded regulator couples the synthesis of toxin and surface structures in Bacillus anthracis. Mol. Microbiol. 2003; 47(4):917-27.
12. Ristroph J.D., Ivins B. Elaboration of Bacillus anthracis antigens in new, defained culture medium. Infect. Immun. 1989;
13. Willams D., Turnbough C. Surface layer protein EA1 is not a component of Bacillus anthracis spores but is a persistent contaminant in spore preparations. J. Bacteriol. 2004; 186(2):566-9.
14. Барков А.М., Липницкий А.В., Евтеева Е.В. Способ получения сыворотки для идентификации возбудителя сибирской язвы. Патент на изобретение № 2191603 от 27.10. 2002 г.
15. Farchaus J.B., Ribot W.J., Downs M., Ezzel J.W. Purification and Charaterization of the major surface array protein from the avirulent Bacillus anthracis Delta Sterne-1. J. Bacteriol. 1995; 177(9):2481-9.
Review
For citations:
Barkova I.A., Alexeev V.V., Lipnitsky A.V., Barkov A.M., Bukhantsova L.V. Production of S-layer proteins by Different Bacillus anthracis Strains. Problems of Particularly Dangerous Infections. 2008;(4(98)):29-32. (In Russ.) https://doi.org/10.21055/0370-1069-2008-4(98)-29-32
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