A Study of Biokinetic Peculiarities and Optimization of the Conditions for Culturing Vibrio cholerae Strains Overproducing Protective Antigens Suitable for Use in the Production
https://doi.org/10.21055/0370-1069-2008-1(95)-52-55
Abstract
Described in the work are the major biokinetic criteria of the submerged growth process of V. cholerae Ol strains (classical and eltor biovars), O139, non-Ol and non-O139 serogroups, capable of overproducing the main protective antigens (Ol and O139 antigens, type I and II cholera toxin, cholera toxin type II В subunit, toxin-coregulated adhesion pili). The optimal parameters were defined for each strain (nutritional media, pH indices, cultivation time, application of an additional carbohydrate source) facilitating the maximal yield of the protective antigens under the production conditions, thus making it possible to use them as a basis for manufacturing more efficient vaccinal preparations as well as to obtain the main V. cholerae protective antigens as purified drugs for constructing diagnostic preparations.
About the Authors
O. A. Volokh
Russian Anti-Plague Research Institute “Microbe”, Saratov
Россия
Россия
I. A. Shepelev
Russian Anti-Plague Research Institute “Microbe”, Saratov
Россия
Россия
S. P. Zadnova
Russian Anti-Plague Research Institute “Microbe”, Saratov
Россия
Россия
I. M. Krepostnova
Russian Anti-Plague Research Institute “Microbe”, Saratov
Россия
Россия
S. A. Yeremin
Russian Anti-Plague Research Institute “Microbe”, Saratov
Россия
Россия
References
1. Громова О.В., Джапаридзе М.Н., Дятлов И.А. и др. Оральная химическая вакцина против холеры. Патент РФ №2159128. - 2000. - 10 с.
2. Джапаридзе М.Н., Никитина Г.П., Куликова В. Л. и др.//ЖМЭИ. - 1984. - №4. - С. 70-74.
3. Заднова С.П., Волох О.А., Крепостнова И.М. и др.//Пробл. особо опасных инф. - 2007. - Вып. 1 (95). - С. 50-55.
4. Смирнова Н.И., Чеховская Г.В., Ливанова Л.Ф. и др.//ЖМЭИ. - 2000. -№3. - С 47-51.
5. Смирнова Н.И., Крепостнова И.М., Ливанова Л.Ф. и др.//Мол. гене. т, микробиол., вирусол. - 2007. - №4. - С. 7-13.
6. Топорков А.В., Заднова С.П., Смирнова Н.И., Кутырев В.В. Штамм бактерий Vibrio cholerae KM206 классического биовара серовара Огава - продуцент протективных антигенов. Патент РФ №2222594. - 2004. - 6 с.
7. Kabir S.//J. Med. Microbiol. - 2005. - Vol. 16. - P. 101-116.
8. Smirnova N.I., Zadnova S.P., Toporkov A.V., Kutyrev V.V.//New Research on Biotechnology and Medicine/Edit. A.M. Egorov, G.E. Zaikov. - New York, 2006. - P. 177-188.
9. Svennerholm A.M., Holmgren J.//Curr.Mcrobiol. - 1978. - Vol. l. - P. 19-23.
10. Towbin H., Stacbelin Т., Gordon J.//Proc. Natl. Acad. Sci. USA. - 1979. - Vol. 76 - P. 4350-4354.
Review
For citations:
Volokh O.A., Shepelev I.A., Zadnova S.P., Krepostnova I.M., Yeremin S.A. A Study of Biokinetic Peculiarities and Optimization of the Conditions for Culturing Vibrio cholerae Strains Overproducing Protective Antigens Suitable for Use in the Production. Problems of Particularly Dangerous Infections. 2008;(1(95)):52-55. (In Russ.) https://doi.org/10.21055/0370-1069-2008-1(95)-52-55
Views:
987
JATS XML
Email this article 