Usage of chi-Sequence for Differentiation between the Strains of Anthrax Etiological Agent and Closely Related Bacilli
https://doi.org/10.21055/0370-1069-2014-2-97-100
Abstract
Objective of the study was to carry out comparison between the microorganisms appurtenant to six different species of Bacilli genus as regards mobility of the amplicons obtained using the primer that contained chi-sequence of Bacillus subtilis . Therewith, analyzed were 30 Bacillus anthracis strains and closely related bacilli. Cultures grew on L-agar and L-broth at 37 °C. DNA was isolated from vegetative B. anthracis and Bacillus spp . cultures introducing lysozyme and phenol de-proteinizing. In order to perform single-primer PCR constructed was ChiBs primer - 5’-CTAGGAGCGGG-3’. Single-primer amplification (30 cycles) was carried out at annealing temperature of 47 °C. Comparative analysis of individual electrophoretic amplicon profiles, obtained by means of ChiBs-primer PCR, allows for identification of genetic intraspecific variability and differentiation of B. anthracis strains from the atypical ones and closely related species of bacilli.
About the Authors
N. A. Shishkova
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
A. N. Mokrievich
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
V. M. Pavlov
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
L. I. Marinin
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
G. M. Vakhrameeva
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
T. Yu. Kudryavtseva
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
I. A. Dyatlov
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
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Review
For citations:
Shishkova N.A., Mokrievich A.N., Pavlov V.M., Marinin L.I., Vakhrameeva G.M., Kudryavtseva T.Yu., Dyatlov I.A. Usage of chi-Sequence for Differentiation between the Strains of Anthrax Etiological Agent and Closely Related Bacilli. Problems of Particularly Dangerous Infections. 2014;(2):97-100. (In Russ.) https://doi.org/10.21055/0370-1069-2014-2-97-100
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