Infection of an Individual with Plague in the Gorno-Altaisk High-Mountain Natural Focus in 2014. Communication 2. Peculiarities of Laboratory Diagnostics and Molecular-Genetic Characterization of the Isolated Strains
https://doi.org/10.21055/0370-1069-2014-4-43-51
Abstract
Laboratory diagnostics of plague was carried out in compliance with valid operational guidelines and regulations. But its peculiarity consisted in the performance of diagnostic investigations secondary to antimicrobial therapy with application of preparations characterized by the expressed activity towards gram-negative microorganisms, including the agent of plague (ceftriaxone, ciprolet, and amikacin). The studies revealed that under antibiotic treatment during the early phase of infection the most effective method for the laboratory plague diagnostics was PCR. Based on the results of the assay it was possible to establish not only provisional, but also the final diagnosis in a patient. Obtained was genetic characteristics of the strains isolated from the patient and the marmot, withdrawn at the patient’s place, using techniques of molecular-genetic analysis, in particular PCR, multilocus VNTR, and multilocus and genome-wide sequencing. Thereupon the strains were attributed to antique biovar of the main subspecies of plague agent. In addition, close relation to Y. pestis of the main subspecies isolated in the same focus in 2012 and to the strains from Mongolian Altai and Tuvinian mountain focus was determined based on phylogenetic analysis of the isolates.
About the Authors
V. V. Kutyrev
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
A. Yu. Popova
Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare
Russian Federation
Russian Federation
E. B. Ezhlova
Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare
Russian Federation
Russian Federation
Yu. V. Demina
Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare
Russian Federation
Russian Federation
N. D. Pakskina
Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare
Russian Federation
Russian Federation
I. N. Sharova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
A. I. Mishchenko
Altai Plague Control Station
Russian Federation
Russian Federation
E. N. Rozhdestvensky
Altai Plague Control Station
Russian Federation
Russian Federation
G. Kh. Bazarova
Altai Plague Control Station
Russian Federation
Russian Federation
E. P. Mikhailov
Altai Plague Control Station
Russian Federation
Russian Federation
G. A. Eroshenko
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
Ya. M. Krasnov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
L. M. Kukleva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
A. V. Cherkasov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
E. G. Oglodin
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
V. E. Kuklev
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
G. N. Odinokov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
S. A. Shcherbakova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
S. V. Balakhonov
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Russian Federation
Russian Federation
M. V. Afanas’Ev
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Russian Federation
Russian Federation
S. A. Vityazeva
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Russian Federation
Russian Federation
M. Yu. Shestopalov
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Russian Federation
Russian Federation
V. T. Klimov
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Russian Federation
Russian Federation
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Review
For citations:
Kutyrev V.V., Popova A.Yu., Ezhlova E.B., Demina Yu.V., Pakskina N.D., Sharova I.N., Mishchenko A.I., Rozhdestvensky E.N., Bazarova G.Kh., Mikhailov E.P., Eroshenko G.A., Krasnov Ya.M., Kukleva L.M., Cherkasov A.V., Oglodin E.G., Kuklev V.E., Odinokov G.N., Shcherbakova S.A., Balakhonov S.V., Afanas’Ev M.V., Vityazeva S.A., Shestopalov M.Yu., Klimov V.T. Infection of an Individual with Plague in the Gorno-Altaisk High-Mountain Natural Focus in 2014. Communication 2. Peculiarities of Laboratory Diagnostics and Molecular-Genetic Characterization of the Isolated Strains. Problems of Particularly Dangerous Infections. 2014;(4):43-51. https://doi.org/10.21055/0370-1069-2014-4-43-51
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