Manufacturing of Hybridomas-Producers of Monoclonal Anti-Bodies to Tularemia Agent Antigens
https://doi.org/10.21055/0370-1069-2015-2-92-94
Abstract
Carried out have been two experimental studies on hybridization of mouse myeloma cells and lymphocytes of BALB/c mice immunized with inactivated microbe Francisella tularensis cultures. As a result obtained have been hybridomas-producers of monoclonal antibodies (MAb) specific to the antigens of tularemia agent. Evaluated have been the prospects of its application for the detection of the agent under discussion using enzyme-linked immunoassay. Established is the fact that monoclonal antibodies produced by 31G1F10, 32E5D3, 35B11C8, 36C2F11 hybridomas make it possible to identify microbe cells of various tularemia agent strains when concentrated up to 0.5·106 mc/sm3, and do not interact with cultures of heterologous microorganisms when concentrated to 1.0·108 mc/sm3, which testifies to their specificity. These MAb are planned to be used for the construction of immune-enzyme and immune-chromatographic test-systems designed for tularemia agent detection.
About the Authors
O. O. Fomenkov
Filial Brunch of the 48th Central Research Institute of the Ministry of Defense of the Russian Federation
Russian Federation
Russian Federation
G. D. Elagin
Filial Brunch of the 48th Central Research Institute of the Ministry of Defense of the Russian Federation
Russian Federation
Russian Federation
G. V. Kuklina
Filial Brunch of the 48th Central Research Institute of the Ministry of Defense of the Russian Federation
Russian Federation
Russian Federation
D. V. Pechenkin
Filial Brunch of the 48th Central Research Institute of the Ministry of Defense of the Russian Federation
Russian Federation
Russian Federation
V. V. Krupin
Filial Brunch of the 48th Central Research Institute of the Ministry of Defense of the Russian Federation
Russian Federation
Russian Federation
N. V. Bogacheva
Filial Brunch of the 48th Central Research Institute of the Ministry of Defense of the Russian Federation
Russian Federation
Russian Federation
A. A. Kytmanov
Filial Brunch of the 48th Central Research Institute of the Ministry of Defense of the Russian Federation
Russian Federation
Russian Federation
A. V. Eremkin
Filial Brunch of the 48th Central Research Institute of the Ministry of Defense of the Russian Federation
Russian Federation
Russian Federation
O. V. Tikhvinskaya
Filial Brunch of the 48th Central Research Institute of the Ministry of Defense of the Russian Federation
Russian Federation
Russian Federation
E. Yu. Vakhnov
Filial Brunch of the 48th Central Research Institute of the Ministry of Defense of the Russian Federation
Russian Federation
Russian Federation
References
1. Bezsmertny V.E., Gorshenko V.V., Popov V.P. [On the assessment of tularemia epidemic and epizootic situation in the Russian Federation]. Probl. Osobo Opasn. Infek. 2008; 2(96):8–12.
2. Nikiforov V.V., Karetkina G.N. [Tularemia: since the discovery to the present day]. Infek. Bol. 2007; 5(1):67–76.
3. Syrova N.A., Tereshkina N.E., Devdariani Z.L. [Current state of tularemia immuno-diagnostics]. Probl. Osobo Opasn. Infek. 2008; 3(97):12–5.
4. Tereshkina N.E., Terekhova I.V., Syrova N.A., Devdariani Z.L., Lyashova O.Yu., Grigor’eva G.V., Lobovikova O.A., Shul’gina I.V., Ivanenko I.L., Zakharova N.B., Bezrukova G.A., Spirin V.F. [Constructing and medical trials of a monoclonal dot-immuno-enzyme test-system “DIATul-M” for tularemia microbe detection]. Probl. Osobo Opasn. Infek. 2013; 2:42–5.
5. Fazekas De St., Groth S., Scheidegger D. Production of monoclonal antibodies: strategy and tactics. J. Immunol. Meth. 1980; 35:1–21.
6. Hauri A.M., Hofstetter I.,Seibold E., Kaysser P., Eckert J., Neubauer H., Splettstoesser W.D., Investigating an airborne tularemia outbreak, Germany. Emerg. Infect. Dis. 2010; 16:238–43.
7. Kohler G., Milstein C. Continuous culture of fused cells secreting antibody of predefined specificity. Nature. 1975; 256(5517):495–7.
8. Willke A., Meric M., Granow R., Sayan M. An outbreak of oropharyngeal tularaemia linked to natural spring water. J. Med. Microbiol. 2009; 1:112–16.
Review
For citations:
Fomenkov O.O., Elagin G.D., Kuklina G.V., Pechenkin D.V., Krupin V.V., Bogacheva N.V., Kytmanov A.A., Eremkin A.V., Tikhvinskaya O.V., Vakhnov E.Yu. Manufacturing of Hybridomas-Producers of Monoclonal Anti-Bodies to Tularemia Agent Antigens. Problems of Particularly Dangerous Infections. 2015;(2):92-94. (In Russ.) https://doi.org/10.21055/0370-1069-2015-2-92-94
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