Improvement of Diagnostic System in a Real-Time RT-PCR “AmpliSens EBOV (ZAIRE)-FL” Format for Zaire ebolavirus RNA Detection
https://doi.org/10.21055/0370-1069-2015-3-55-57
Abstract
Taking into consideration experience in utilization of “AmpliSens EBOV (ZAIRE)-FL” in the setting of SAET mobile laboratory complex, carried out has been the improvement of the panel with a view to elapsed time reduction and analytical sensitivity enhancement. It provides for the analytical sensitivity increase up to 200 copies per ml, which is 10 times higher than the previous measurement, that accounted for 2·103 copies per ml. The timing of the assay has been reduced up to 1 h 25 min, which is 25 % less than the time needed for the standard study. Modification of “AmpliSens EBOV (ZAIRE)-FL” panel benefits to the enhancement of epidemiological surveillance activities intended to stop the spread of Zaire ebolavirus in West Africa and prevent its importation into the Russian Federation.
About the Authors
V. G. Dedkov
Central Research Institute of Epidemiology; RAS Research Institute of Occupational Medicine
Russian Federation
Russian Federation
M. V. Safonova
Central Research Institute of Epidemiology
Russian Federation
Russian Federation
S. A. Bodnev
State Research Centre of Virology and Biotechnology “Vector”
Russian Federation
Russian Federation
A. S. Kabanov
State Research Centre of Virology and Biotechnology “Vector”
Russian Federation
Russian Federation
V. A. Safronov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
A. A. Lopatin
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
V. E. Kuklev
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
D. V. Utkin
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
V. V. Maleev
Central Research Institute of Epidemiology
Russian Federation
Russian Federation
G. A. Shipulin
Central Research Institute of Epidemiology
Russian Federation
Russian Federation
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For citations:
Dedkov V.G., Safonova M.V., Bodnev S.A., Kabanov A.S., Safronov V.A., Lopatin A.A., Kuklev V.E., Utkin D.V., Maleev V.V., Shipulin G.A. Improvement of Diagnostic System in a Real-Time RT-PCR “AmpliSens EBOV (ZAIRE)-FL” Format for Zaire ebolavirus RNA Detection. Problems of Particularly Dangerous Infections. 2015;(3):55-57. (In Russ.) https://doi.org/10.21055/0370-1069-2015-3-55-57
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