Characteristics of Molecular-Genetic Properties of Plague Agent Strains - Promising for Application with Educational Purposes
https://doi.org/10.21055/0370-1069-2016-2-83-86
Abstract
Training of specialists in advanced methods of plague laboratory diagnostics and in the safety of works with its agent is associated with the necessity to select strains for educational purposes, allowing for the reduction of risk of laboratory infection at tutorials. Deployment of avirulent strains or those with attenuated virulence is the guiding principle in the case. Objective of the study is to detect basic virulence genes of chromosomal and plasmid localization in plague agent strains - candidates to be used for educational purposes. Materials and methods. Utilized have been 18 strains of Yersinia pestis . Investigations have been carried out with the help of reagent panel for identification of Y. pestis strains using real-time polymerase chain reaction (RT-PCR) with hybridization-fluorescent registration of results (“Gene Yersinia pestis Identification - HFR”). Results and discussion. In the course of the study, the strains, selected as the prospective ones, have been characterized by composition of the key virulence genes of chromosomal and plasmid localization. The operation has been performed by means of the reagent panel for identification of Y. pestis strains using RT-PCR with hybridization-fluorescent registration of results. Consequently to the assessment of molecular-genetic properties of plague agent strains, distinguished have been genetic variants (the genome of which lacks one or more key virulence determinants) which are the most relevant in the reduction of biological risks of training technologies at tutorials within the frames of the educational course “Microbiology and laboratory diagnostics of plague”.
About the Authors
E. V. Sazanova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
V. E. Kuklev
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
T. A. Malyukova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
L. M. Kukleva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
A. N. Malakhaeva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
N. I. Vakhrushina
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
References
1. [Conception of the Federal Target Program “National system of chemical and biological safety of the Russian Federation”]. (Cited 11 Mar 2016). Available from: http://fcgie.ru/DOC/170712/post_74r.pdf.
2. Kutyrev V.V., Popova A.Yu., Ezhlova E.B., Demina Yu.V., Pakskina N.D., Shchuchinov L.V., Mikhailov E.P., Mishchenko A.I., Rozhdestvensky E.N., Bazarova G.Kh., Denisov A.V., Sharova I.N., Popov N.V., Kuznetsov A.A. [Infection of an individual with plague in the Gorno-Altaisk highmountain natural focus in 2014. Communication1. Epidemiological and epizootiological peculiarities of plague manifestations in the Gorno-Altaisk high-mountain (Sailyugemsky) natural plague focus]. Probl. Osobo Opasn. Infek. 2014; 4:9–16.
3. Kutyrev V.V., Protsenko O.A. [Classification and molecular-genetic investigations of Yersinia pestis]. Probl. Osobo Opasn. Infek. 1998; 78:11–22.
4. Onishchenko G.G., Kutyrev V.V., editors. [Laboratory diagnostics of dangerous infectious diseases: practice guidelines. 2nd edition]. M.: “Shiko”; 2013. 560 p.
5. [Organization and carrying out of epidemiological surveillance in the natural plague foci in the territory of the Russian federation]. MR 3.1.3.2355-08. M.; 2009. 103 p.
6. [Basic Principles of the State Policy in the provision of chemical and biological safety of the Russian Federation for the period until 2025 and prospective further on]. (Cited 11 Mar 2016). Available from: http://docs.cntd.ru/document/499057916.
7. Popov Yu.A., Protsenko O.A., Anisimov P.I., Kokushkin A.M., Mozharov O.T. [Detection of pesticinogenicity plasmids in plague microbe using agarose gel electrophoresis]. In: [Prophylaxis of Particularly Dangerous Infections]. Saratov; 1980. P. 20–5.
8. [Procedure of organization and carrying out of laboratory diagnostics of plague for the territorial, regional, and federal facilities]. MR 4.2.2940-11. M; 2011. 55 p.
9. [Preparation of samples with surrogates of pathogenic biological agents]. MR 4.2.1103-02. M.; 2002.
10. Protsenko O.A., Anisimov P.I., Mozharov O.T., Konnov N.P., Popov Yu.A., Kokushkin A.M. [Detection and characteristics of plague microbe plasmids, determining synthesis of pesticin I, antigen fraction I, and exotoxin of “murine toxin”]. Genetika. 1983; 19(7):1081–90.
11. Sazanova E.V., Malyukova T.A., Popov Yu.A. [Dummy Yersinia pestis strains: selection criteria, usage guidelines]. Probl. Osobo Opasn. Infek. 2014; 3:38–41.
12. Hinnebusch B.J., Rudolph A.E., Cherepanov P., Dixon J.E., Schwan T.G., Forsberg A. Role of Yersinia murine toxin in survival of Yersinia pestis in the midgut of the flea vector. Science. 2002; 296:733–35. DOI: 10.1126/science.1069972.
13. Kado C.I., Liu S.T. Rapid procedure for detection and isolation of large and small plasmids. J. Bacteriol. 1981; 145 (3):1365–73.
14. Kutyrev V.V., Mehigh R.J., Motin V.L., Pokrovskaya M.S., Smirnov G.B., Brubaker R.R Expression of the plague plasminogen activator in Yersinia pseudotuberculosis and Escherichia coli. Infect. Immun. 1999; 67:1359–67.
15. Perry R.D., Fetherston J.D. Yersinia pestis – etiologic agent of plague. Clin. Microbiol. Rev. 1997; 10:35–66.
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Review
For citations:
Sazanova E.V., Kuklev V.E., Malyukova T.A., Kukleva L.M., Malakhaeva A.N., Vakhrushina N.I. Characteristics of Molecular-Genetic Properties of Plague Agent Strains - Promising for Application with Educational Purposes. Problems of Particularly Dangerous Infections. 2016;(2):83-86. (In Russ.) https://doi.org/10.21055/0370-1069-2016-2-83-86
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