Comparative MLVA-Analysis of Vibrio cholerae Strains of Classical Biovar, Isolated in the Russian Federation and Abroad
https://doi.org/10.21055/0370-1069-2016-4-88-92
Abstract
Objective of the study is to determine phylogenetic affinity of the Vibrio cholerae strains of classical biovar, isolated between 1937 and 1969 in Russia, to the strains from near and far abroad countries. Materials and methods. Utilized were 27 Vibrio cholerae strains of classical biovar. PCR was carried out applying “BIS M112” amplifier. Genetic analyzer ABI 3500xl was used for sequencing of the strains. MLVA-analysis was performed by reference to 5 MLVA-loci: VC0147, VC0436-0437, VC1650, VCA0171, and VCA0238. Nutrient requirement and soluble hemagglutinin/protease production was established using plate method. Results and conclusions. Identified have been 8 MLVA-clusters and 21 MLVA-types. It is determined that the strains, isolated during atypical cholera outbreak 1942–1943 in Russia, are inhomogeneous as regards phenotype and genotype and fall into two separate groups, one of which is related to the strain, isolated during cholera outbreak 1938 in Khabarovsk, and another group – to the strains from India and China, isolated in 1946 and 1949, respectively.
About the Authors
N. B. Cheldyshova
Russian Research Anti-Plague Institute “Microbe”, Saratov
Russian Federation
46, Universitetskaya St., Saratov, 410005
Russian Federation
46, Universitetskaya St., Saratov, 410005
A. A. Kritsky
Russian Research Anti-Plague Institute “Microbe”, Saratov
Russian Federation
46, Universitetskaya St., Saratov, 410005
Russian Federation
46, Universitetskaya St., Saratov, 410005
Yu. V. Lozovsky
Russian Research Anti-Plague Institute “Microbe”, Saratov
Russian Federation
46, Universitetskaya St., Saratov, 410005
Russian Federation
46, Universitetskaya St., Saratov, 410005
N. P. Guseva
Russian Research Anti-Plague Institute “Microbe”, Saratov
Russian Federation
46, Universitetskaya St., Saratov, 410005
Russian Federation
46, Universitetskaya St., Saratov, 410005
References
1. Vodop’yanov A.S., Vodop’yanov S.O., Mishan’kin M.B., Suchkov I.Yu. [Variable tandem repeats, detected by means of computer-based analysis of Vibrio cholerae genome]. Biotekhnologiya. 2001; 6:85–8.
2. Pokrovsky V.I., editor. [Cholera in the USSR during the VII Pandemic]. M.; 2000. 472 p.
3. Smirnova N.I., Kul’shan’ T.A., Krasnov Ya.M. [MLVA-typing of clinical Vibrio cholerae strains, isolated in different periods of the current cholera pandemic]. Mol. Genet. Mikrobiol. Virusol. 2015; 1:15–22. DOI: 10.3103/S0891416815010085.
4. Smirnova N.I., Cheldyshova N.B., Zadnova S.P., Kutyrev V.V. [Molecular-genetic peculiarities of Vibrio cholerae classica strains, which caused Asian cholera pandemic in Russia in 1942]. Mol. Genet. Mikrobiol. Virusol. 2001; 4:12–6.
5. Cheldyshova N.B., Kritsky A.A., Krasnov Ya.M., Smirnova N.I. [Analysis of the results of fragment and whole genome sequencing of atypi- cal Vibrio cholerae strains, classical biovar, which caused Asian cholera pan- demic in Russia]. Epidemiol. Infek. Bol. 2015; 20(5):24–31.
6. Alam M., Islam M.T., Rashed S.M., Johura F.T., Bhuiyan N.A., Delgado G., Morales R., Mendez J.L., Navarro A., Gil A.I., Watanabe H., Morita M., Nair B., Cravioto A. Vibrio cholerae classical biotype strains re- veal distinct signatures in Mexico. J. Clin. Microbiol. 2012; 50 (7):2212–6. DOI:10.1128/JCM.00189-12.
7. Bakhshi B., Boustanshenas M., Mahmoudi-Аznaveh A. Emergence of Vibrio cholerae O1 classical biotype in 2012 in Iran. Lett. Appl. Microbiol. 2014; 58 (2):145–9. DOI:10.1111/lam.12167.
8. Choi S.Y., Lee J.H., Jeon Y.S., Lee H.R., Kim E.J., Ansaruzzaman M., Bhuiyan N.A., Endtz H.P., Niyogi S.K., Sarkar B.L., Nair G.B., Nguen B.M., Hien N.T., Czerkinsky C., Clemens J.D., Chun J., Kim D.W. Multilocus variable-number tandem repeat analysis of Vibrio cholerae O1 El Tor strains harbouring classical toxin B. J. Med. Microbiol. 2010; 59(7):763–9. DOI: 10.1099/jmm.0.017939-0.
9. Dziejman M., Balon E., Boydet D., Fraser C.M., Heidelberg J.F., Mekalanos J.J. Comparative genomic analysis of Vibrio cholerae: genes that correlate with cholera endemic and pandemic disease. Proc. Natl. Acad. Sci. 2002; 99:1556–61. DOI: 10.1073/pnas.042667999.
10. Hase C.C., Finkelstein R.A. Cloning and nucleotide sequence of the Vibrio cholerae hemagglutinin/protease (HA/Protease) gene and construc- tion of an HA/Protease-negative strain. J. Bacteriol. 1991; 173:3311–17.
11. Kokashvili T, Elbakidze T, Jaiani E, Janelidze N, Kamkamidze G, Whitehouse C., Huq A., Tediashvili M. Comparative phenotypic character- ization of Vibrio cholerae isolates collected from aquatic environments of Georgia. Georgian Med. News. 2013; 224:55–62.
12. Longini I.M. Jr., Yunus M., Zaman K., Siddique A.K., Sack R.B., Nizam A. Epidemic and endemic cholera trends over a 33-year period in Bangladesh. J. Infect. Dis. 2002; 186(2):246–51. DOI: 10.1086/341206.
13. Pun S.B. The first appearance of classical-like phenotype Vibrio cholerae in Nepal. N. Am. J. Med. Sci. 2014; 6(4):183–4. DOI: 10.4103/1947- 2714.131248.
14. Rai K.R, Rai S.K, Bhatt D.R, Kurokuwa M, Ono K, Magar D.T. Study of medically important Vibrios in the sewage of Katmandu Valley, Nepal. Nepal Med. Coll. J. 2012; 14(3):212–5.
Review
For citations:
Cheldyshova N.B., Kritsky A.A., Lozovsky Yu.V., Guseva N.P. Comparative MLVA-Analysis of Vibrio cholerae Strains of Classical Biovar, Isolated in the Russian Federation and Abroad. Problems of Particularly Dangerous Infections. 2016;(4):88-92. (In Russ.) https://doi.org/10.21055/0370-1069-2016-4-88-92
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