AUTHENTICATION OF REFERENCE STRAINS OF PATHOGENIC MICROORGANISMS APPLYING AUTOMATED MICROBIOLOGICAL ANALYZER “VITEK 2”
https://doi.org/10.21055/0370-1069-2017-1-100-104
Abstract
Objective of the study was to carry out identification of reference strains of pathogenic microorganisms from stores of the State Collection of Pathogenic Bacteria (SCPB) at RusRAPI “Microbe”, using microbiological analyzer Vitek 2, followed by determination of their biochemical authenticity markers.
Materials and methods. Methodological approach was based on application of automated system Vitek 2 Bio-Mérieux with the subsequent assessment of the results obtained, using BioNumerics 7.5 software package.
Results and conclusions. Verified was authenticity of 103 reference strains of pathogenic microorganisms stored in SCPB, RusRAPI “Microbe”. It was established that 7 out of 103 strains (6.8 %) did not conform to the claimed feature profiles and cannot be used in scientific-practical activities, in consequence of which they were replaced with reliable authentic analogues. Evaluation of the data on interaction between the tested strains and differentiating substrates allowed for determination of authenticity markers for each of them, necessary for control of collection culture identity under reproduction and storage. On the basis of the data gathered, the profiles of the studied reference strains were regenerated.
About the Authors
N. S. Chervyakova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
46, Universitetskaya St., Saratov, 410005, e-mail: rusrapi@microbe.ru
Russian Federation
46, Universitetskaya St., Saratov, 410005, e-mail: rusrapi@microbe.ru
A. V. Osin
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
46, Universitetskaya St., Saratov, 410005, e-mail: rusrapi@microbe.ru
Russian Federation
46, Universitetskaya St., Saratov, 410005, e-mail: rusrapi@microbe.ru
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Review
For citations:
Chervyakova N.S., Osin A.V. AUTHENTICATION OF REFERENCE STRAINS OF PATHOGENIC MICROORGANISMS APPLYING AUTOMATED MICROBIOLOGICAL ANALYZER “VITEK 2”. Problems of Particularly Dangerous Infections. 2017;(1):100-104. (In Russ.) https://doi.org/10.21055/0370-1069-2017-1-100-104
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