Evaluation of Effectiveness of Yersinia pestis Molecular Diagnostics in the Field Material from Sarydzhas Focus in Kyrgyzstan
https://doi.org/10.21055/0370-1069-2017-4-23-26
Abstract
Objective of the study is to assess the effectiveness of the methods for molecular diagnostics and identification of Yersinia pestis strains in the field material obtained from Sarydzhas high-mountain focus in Kyrgyz Republic. Materials and methods. Investigated were the samples of the field material, isolated in 2016 in Sarydzhas high-mountain plague focus, using conventional methods of laboratory diagnostics and PCR with hybridization-fluorescent and electrophoretic registration of results. Results and conclusions. It is demonstrated that in a number of cases molecular-genetic method has a higher resolution as compared to conventional methods of laboratory diagnostics of plague. It proves the necessity of wider usage of molecular-diagnostic methods in epizootiological monitoring of plague in natural foci in Kyrgyz Republic.
About the Authors
A. K. Dzhaparova
Republican Center on Quarantine and Particularly Dangerous Infections of the Ministry of Health of the Kyrgyz Republic
Kyrgyzstan
Kyrgyzstan
92, Skryabina St., Bishkek, Kyrgyz Republic
S. T. Abdikarimov
Republican Center on Quarantine and Particularly Dangerous Infections of the Ministry of Health of the Kyrgyz Republic
Kyrgyzstan
Kyrgyzstan
92, Skryabina St., Bishkek, Kyrgyz Republic
G. A. Eroshenko
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
46, Universitetskaya St., Saratov, 410005, Russian Federation
Russian Federation
46, Universitetskaya St., Saratov, 410005, Russian Federation
N. Yu. Nosov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
46, Universitetskaya St., Saratov, 410005, Russian Federation
Russian Federation
46, Universitetskaya St., Saratov, 410005, Russian Federation
L. M. Kukleva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
46, Universitetskaya St., Saratov, 410005, Russian Federation
Russian Federation
46, Universitetskaya St., Saratov, 410005, Russian Federation
D. A. Adambekov
I.Akhunbaev Kyrgyz State Medical Academy
Kyrgyzstan
92, Akhunbaeva St., Bishkek, Kyrgyz Republic
Kyrgyzstan
92, Akhunbaeva St., Bishkek, Kyrgyz Republic
I. Sh. Al’dzhambaeva
I.Akhunbaev Kyrgyz State Medical Academy
Kyrgyzstan
92, Akhunbaeva St., Bishkek, Kyrgyz Republic
Kyrgyzstan
92, Akhunbaeva St., Bishkek, Kyrgyz Republic
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For citations:
Dzhaparova A.K., Abdikarimov S.T., Eroshenko G.A., Nosov N.Yu., Kukleva L.M., Adambekov D.A., Al’dzhambaeva I.Sh. Evaluation of Effectiveness of Yersinia pestis Molecular Diagnostics in the Field Material from Sarydzhas Focus in Kyrgyzstan. Problems of Particularly Dangerous Infections. 2017;(4):23-26. (In Russ.) https://doi.org/10.21055/0370-1069-2017-4-23-26
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