Development of the PCR Assay with Universal Primers for the Detection of Different tcpA Gene Variants
https://doi.org/10.21055/0370-1069-2012-1(111)-102-103
Abstract
Developed is the PCR assay for the detection of the structural genes of toxin co-regulated adhesion piluses - tcpA of different types. Determined are the universal primers, the usage of which provides for the detection of the stated above genes in V. cholerae of various serogroups. With the help of this PCR assay identified is a new variant of tcpA gene in toxigenic cholera vibrio of non-O1/non-O139 serogroup.
About the Authors
A. V. Fadeeva
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
G. A. Eroshenko
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
G. N. Odinokov
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
N. A. Sharapova
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
T. V. Valova
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
V. V. Kutyrev
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
References
1. Bertran P., Delgado G., Navarro A., Truhillo F., Selander R.K., Gravioto A. Genetic diversity and population structure of Vibrio cholerae. J. Clin. Microbiol. 1999; 37:581-90.
2. Keasler S.P., Hall R.H. Detecting and biotyping Vibrio cholerae O1 with multiplex polymerase chain reaction. Lancet. 1993; 341:1661.
3. Nandi B., Nandy R.K., Vicente A.C., Ghose A.C. Molecular characterization of a new variant of toxin-coregulated pilus protein (tcpA) in a toxigenic non-O1/non-O139 strain of Vibrio cholerae. Infect. Immun. 2000; 68:948-52.
4. Taylor R.K., Miller V.L, Furlong D.B. Mekalonos J.J. Use of phoA gene fusion to identify a pilus colonization factor coordinately regulated with cholera toxin. Proc. Natl. Acad. Sci. USA. 1987; 84:2833-7.
Review
For citations:
Fadeeva A.V., Eroshenko G.A., Odinokov G.N., Sharapova N.A., Valova T.V., Kutyrev V.V. Development of the PCR Assay with Universal Primers for the Detection of Different tcpA Gene Variants. Problems of Particularly Dangerous Infections. 2012;(1(111)):102-103. (In Russ.) https://doi.org/10.21055/0370-1069-2012-1(111)-102-103
Views:
583
Email this article 