Studies of Biofilm Formation in Non-Pigmented and Plasmid-Deprived Mutants of Yersinia pestis on Biotic Surfaces, in vivo and in vitro Conditions
https://doi.org/10.21055/0370-1069-2012-3-45-49
Abstract
Yersinia pestis 231 strain – studied is the mechanism of biofilm formation on biotic surfaces, both
in vitro (on the laboratory model of nematode
Caenorhabdiitis elegans) and
in vivo (inside the alimentary tract of
Nosopsyllus laeviceps flea). It is determined that spontaneous loss of ability to form biofilms and generate pigmented colonies in the mutants is probably caused not only by the deletion of the whole chromosome pigmentation fragment, but also by a point(single base) mutation in structural
hms operon. It is demonstrated that the absence of
pCad, pFra or
pPst plasmids does not have an impact on the ability of plasmid-deprived mutants to form biofilm on the cuticle of nematode
C. elegans.
About the Authors
G. A. EroshenkoRussian Federation
N. A. Vidyaeva
Russian Federation
L. M. Kukleva
Russian Federation
E. I. Koshel’
Russian Federation
G. N. Odinokov
Russian Federation
N. Yu. Shavina
Russian Federation
T. V. Knyazeva
Russian Federation
T. V. Mokrousova
Russian Federation
Ya. M. Krasnov
Russian Federation
L. V. Anisimova
Russian Federation
L. A. Novichkova
Russian Federation
P. S. Erokhin
Russian Federation
A. V. Boiko
Russian Federation
V. V. Kutyrev
Russian Federation
References
1. Velichko L.N., Knyazeva T.V., Anisimov A.P., Nazarova L.S., Mokrousova T.V., Kokushkin A.M. et al. [Effect of Fra gene expression of Yersinia pestis operon on the vector-borne and alimentary routes of plague transmission by rodents]. Probl. Osobo Opasn. Infek. 2000; 80:127–32.
2. Velichko L.N., Kokushkin A.M., Knyazeva T.V., Mokrousova T.V., Marysaev V.B. [Usage of the equipment for work with plague infected fleas]. Probl. Osobo Opasn. Infek. 1994; 4:49–55.
3. Kokushkin A.M., Velichko L.N., Knyazeva T.V., Anisimov P.I., Mokrousova T.V. [Studies of the impact of intrinsic (inherent) plasmids and pigment-sorption characteristic on the plague agent ability to infect fleas and initiate block-formation in them]. In: [Natural Foci Infections and Their Prophylaxis]. Saratov; 1991. P. 110–8.
4. Kutyrev V.V., Eroshenko G.A., Kukleva L.M., Shavina N.Yu., Vinogradova N.A. [Comparative genetic characterization of the vac- cine Yersinia pestis EV strain and its potential “virulent derivatives”]. Zh. Mikrobiol. Epidemiol. Immunobiol. 2009; 4:50–6.
5. Maniatis T., Frich E., Sambruk G. [Molecular cloning]. M., 1984. 480 p.
6. Bacot A.W., Martin C.J. Observation on the mechanism on the transmission of by fleas. J. Hyg. Plague Suppl. 1914; 3:423–39.
7. Bobrov A.G., Kirillina O., Ryjenkov D.A., Waters C.M., Price P.A., Fetherston J.D. et al. Systemic analysis of cyclic di-GNP signaling enzymes and their role in biofilm formation and virulence in Yersinia pestis. Mol. microbiol. 2011; 79:533–51.
8. Darby C, Hsu J.W., Ghori N., Falkow S. Caenorhabditis elegans: plague bacteria biofilm blocks food intake. Nature. 2002; 417:243–4.
9. Darby C, Ananth S.L., Tan L., Hinnebusch B.J. Identification of gmhA, a Yersinia pestis gene required for flea blockage, by using a Caenorhabditis elegans biofilm system. Infect. Immun, 2005; 73:7236–42.
10. Eroshenko G.A., Vidyaeva N.A., Kutyrev V.V. Comparative analysis of biofilm formation by main and nonmain subspecies of Yersinia pestis strains. FEMS Immunol. Med. Microbiol. 2010; 59:513–20.
11. Fetherston J.D., Schuetze, P., Perry, R.D. Loss of the pigmentation phenotype in Yersinia pestis is due to the spontaneous deletion of 102 kb of chromosomal DNA which is flanked by a repetitive element. Mol. Microbiol. 1992; 6:512693–704.
12. Jackson S., Burrows T. The pigmentation of Pasteurella pestis on a defined medium containing haemin. Brit. J. Exp. Pathol. 1956; 37:570–6.
13. Kirillina O., Fetherston J., Bobrov A., Abney J., Perry R. HmsP, a putative phosphodiesterase, and HmsT, a putative diguanylate cyclase, control Hms-dependent biofilm formation in Yersinia pestis. Mol. Microbiol. 2004; 54:75–88.
14. Kutyrev V.V., Filippov A.A., Oparina O.S., Protsenko O.A. Analysis of Yersinia pestis chromosomal determinants Pgm+ and Psts associated with virulence. Microb. Pathog. 1992; 12(3):177–86.
15. Patel C.N., Wortham B.W., Lines J.L., Fetherston J.D., Perry R.D., Oliveira M.A. Polyamines are essential for the formation of plague biofilm. J. Bacteriol. 2006; 188:2355–63.
16. Vadivaloo V.V. PhoP regulation of Yersinia pestis during flea infection. In: «Yersinia 2010». 10 th. Intern. Symp.; Oct. 23–27, 2010; Brazil. P. 67.
Review
For citations:
Eroshenko G.A., Vidyaeva N.A., Kukleva L.M., Koshel’ E.I., Odinokov G.N., Shavina N.Yu., Knyazeva T.V., Mokrousova T.V., Krasnov Ya.M., Anisimova L.V., Novichkova L.A., Erokhin P.S., Boiko A.V., Kutyrev V.V. Studies of Biofilm Formation in Non-Pigmented and Plasmid-Deprived Mutants of Yersinia pestis on Biotic Surfaces, in vivo and in vitro Conditions. Problems of Particularly Dangerous Infections. 2012;(3(113)):45-49. (In Russ.) https://doi.org/10.21055/0370-1069-2012-3-45-49