Studies of Biofilm Formation in Non-Pigmented and Plasmid-Deprived Mutants of Yersinia pestis on Biotic Surfaces, in vivo and in vitro Conditions

In non-pigmented and plasmid-deprived mutants – isogenic variants of highly virulent
Yersinia pestis 231 strain – studied is the mechanism of biofilm formation on biotic surfaces, both
in vitro (on the laboratory model of nematode
Caenorhabdiitis elegans) and
in vivo (inside the alimentary tract of
Nosopsyllus laeviceps flea). It is determined that spontaneous loss of ability to form biofilms and generate pigmented colonies in the mutants is probably caused not only by the deletion of the whole chromosome pigmentation fragment, but also by a point(single base) mutation in structural
hms operon. It is demonstrated that the absence of
pCad, pFra or
pPst plasmids does not have an impact on the ability of plasmid-deprived mutants to form biofilm on the cuticle of nematode
C. elegans.

возбудитель чумы, мутанты, биопленка, биотическая поверхность, plague agent, mutants, biofilm, biotic surface

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