Considered are the changes introduced into the list of infections, which require implementation of measures for the provision of sanitary protection of the territories, formalized in the international and state regulatory documents. Analyzed are the basic criteria for assigning infections to the category of – “requiring sanitary control”. Distinguished are the two groups of criteria for assigning infectious diseases to the group of infections that require implementation of measures for the provision of sanitary protection of the territories, either on the country-wide level or within the frames of interstate alliances.

Outlined is a brief characteristics on epizootic and epidemic activity of tularemia natural foci in the territory of the Central Federal district of the Russian Federation over a period of 1992–2012. Implementation of GIS-technologies has made it possible to design retrospective map of the sites of the district population infectioning in 2005, and to distinguish the regions that are of a great epidemic significance.

The following factors have been used to characterize 12 species and subspecies of fleas (Siphonaptera) and plague microbe (Yersinia pestis subs. pestis): the proportion of fleas with “blocks”, “blockules” and their ratio, called the index of microbe aggregation. The block formation is registered in 10, whereas formation of blockules are registered in all of the studied species and subspecies of Siphonaptera , the difference between active and passive transmitters according to the last characteristics being unreliable. The fleas, among of which “blocked” ones are not revealed in the experiments, are capable to transmit the causative agent of plague, sometimes with generalization of infectious process in animals, that can provide for continuous transmission of microbe without formation of “blocks” in the proventriculus. Thus, the high value of “rate of block formation” does not reflect the existence of coevolution in the interactions between microbe and its transmitter.

Submitted are the materials on epizootiology of West Nile Fever in birds, taking into account their migration. Described are the characteristics of WNF in wild and synanthropic birds. Ecological relationships between birds, mosquitoes, ticks and West Nile virus are analyzed. Main autumn bird migratory directions are characterized and the data on WNF monitoring in birds in different geographical regions of Russia are presented.

Carried out is the analysis of epizootiological and epidemiological situation on anthrax in the Republics of Altai, Buryatia and Tuva and the Trans-Baikal Region bordering Mongolia for the period of 1985–2010. The Republics of Buryatia and Tuva, and the Trans-Baikal Region are the territories with unsafe epizootiological and epidemiological situation, while the Altai Republic is relatively safe territory in this regard. Long extension of frontiers, intensification of trade and economic cooperation, passenger and cargo traffic increase, and existence of soil foci predetermine the necessity of inter-state partnership in monitoring these foci and making forecast on anthrax epizootiological and epidemiological situation.

Put forward is the method of usage of Arc GIS 10 software product for epidemiological surveillance over brucellosis in the Stavropol region (SR). Designed is the structure of databases on epidemiology and epizootiology of brucellosis in the constituent entity of the Russian Federation. Constructed are two databases (Epidemiology of brucellosis in the SR and Epizootiology of brucellosis in the SR) compatible with Arc GIS 10. Carried out is the zoning of the SR territories based on assessment of the risk of infecting with brucellosis, using Arc GIS computer program.

Analyzed is the performance of works with the strains of fixed rabies virus under the conditions of anti-rabies immunoglobulin manufacturing. Evaluated is the effectiveness of the established system for the provision of biological safety, which has allowed for safe manufacturing of anti-rabies immunoglobulin within the period of 12 years.

The preference of the (21±1) °C temperature for cultivation of the vaccine strain Yersinia pestis EV in the live plague vaccine biomass production to (27±1) °C is experimentally confirmed. An original interpretation of the mechanism of small cells stability that are formed at (21±1) °C has been proposed. This interpretation stipulates a smaller damaging effect of steam molecules during sublimation in the process of lyophilization.

Submitted are the data of examination of the ultrastructure of two phenotypically different clones (toxigenic and non-toxigenic) of V. cholerae Dakka35 strain by means of electron microscopy. Additional layer (capsule) of polysaccharide nature is identified on the surface of non-toxigenic clone bacteria. This layer (absent in toxigenic clone) alters colony morphology.

Displayed is the possibility of differentiation between typical and genetically altered strains of Vibrio cholerae biovar El Tor, which vary in their virulence and epidemic potential, by means of MLVA. Determined is a significant genetic diversity of the gene-variants, probably, due to their polyclonal origin and continuous alterations within genome under the influence of varying environmental factors.

Studied are Vibrio cholerae of different serogroups on the presence of cef (CHO cell elongating factor) gene and activity against tweens and tributyrin using HDS-agar, prepared on the basis of bakery yeast pancreatic digest. Determined is the fact that all cef-positive strains hydrolyze tweens 20, 40, 60, 80, 85 and all but toxigenic V.cholerae O139, hydrolyze tributyrin. In contrast, non-toxigenic cef-negative strains of O139 serogroup, are active only against the latter. Apparently, the tweenase activity of Vibrio cholerae is provided, partly, by Cef, and the ability to hydrolyze tributyrin is the result of combined activity of Cef and other ferments. Shown is the efficiency of HDS-agar application to determine these characteristics.

Variable chromosome loci have been searched, that differentiate Yersinia pestis strains of medievalis from antiqua biovars. The strains of medievalis biovar are shown to possess two deletions – 183 and 70 bps. The first deletion, localized in the inter-gene region aceA – aceK is present in the majority of medievalis biovar strains, the second one, in the gene y1694 , is determined in all medievalis biovar strains isolated in the territory of Russia and neighboring countries.

Carried out is the analysis of genetic peculiarities of Yersinia pestis strains (main ssp.), isolated in natural foci of the Russian Federation and Mongolia. Determined is the fact that strains of medieval biovar characterized by typical glpD1 napA2 rhaS1 genotype circulate in 7 out of 9 marmot and sandy-type foci. Strains of antique biovar characterized by glpD1 napA1 rhaS1 genotype prevail in the Trans-Baikal steppe foci. Y. pestis strains from Tuva mountain focus have similar genotype, which correlates with denitrification activity and absence of marker mutation of medieval biovar – single nucleotide substitution G→T in the 613 position of napA gene, and testifies to affiliation of these strains to antique biovar. Among Y. pestis strains isolated in Mongolia, identified are two atypical strains incapable of reducing nitrates but pertinent to antique biovar.

The comparative experiments demonstrated strong correlation between the index of Yersinia pestis EV NIIEG strain adhesion to human red blood cells and hydrophobic properties of bacteria. At the same time no dependence between hemagglutination and adhesion of plague microbe was determined. Bacterial culture treatment with trypsin and high temperature (56 °C) decreased adhesion. Incubation of microbial cells with red blood cells in the presence of antibiotics (ampicillin, gentamycin, doxycycline and cefotaxime) had no influence on their interaction. The results of investigations suggested the major role of hydrophobic interactions in Y.pestis EV NIIEG strain adhesion to human red blood cells provided by the surface structures of microbial cells of the lipoprotein origin.

, rstC genes), and 7 strains – to the genotype I. Genotypes I and II (genetically altered ones) are the hybrid variants of the El Tor biovar producing CT of the first type.

The phenotypic and molecular-genetic analysis of
Vibrio cholerae O1 El Tor Inaba strain 301, isolated in 2011 from sea water in recreation zone in Taganrog, demonstrated this isolate to be a genetic variant of El Tor cholera causative agent. Its genome was shown to carry a hybrid prophage, containing gene ctxB of classical type and gene rstR of El Tor type, as well as altered pathogenicity island VPI-1 and pandemicity island VSP-II. This strain produced much more cholera toxin (0,12 mcg/ml) than typical strains of this causative agent.

week after immunization (specific activity is identified using neutralization reaction on the model of white mice and dot-blot immunoassay). This level of activity is sufficient for the fractioning of immune serum and extraction of anti-rabies immunoglobulin. Physicochemical and biological properties of the anti-rabies immunoglobulin, obtained with the help of cultural antigen technique, meet the requirements stated in the normative documentation on anti-rabies immunoglobulins extracted from equine blood serum. Specific activity level of experimental batches of anti-rabies immunoglobulin, obtained with the help of cultural technologies, corresponds to 242 and 214 IU/ml.

Studied is the anti-cholera activity of 40 strains from different species of probiotic bacteria according to the main qualitative characteristics, which are determined on the basis of quantitative data. Established are the selection criteria for probiotic bacteria to be used for cholera treatment: antagonistic activity against
V. cholerae – high or moderate, adhesion – only moderate, acid forming activity – high or moderate. The preventive and medical efficiency of 5 selected strains of lactobacilli and 1 strain of bifidobacterium is demonstrated on the experimental model of cholera. Underlined is the prospective use of probiotics as additional preparations of choice against cholera infection.

lymphocytes) isolated from mice, immunized against tularemia. Put forward is the proposition to use tularin in serological surveys and reactions
in vitro for identification of postinfectious or postvaccinal immunity to tularemia.

Evaluated are immunobiological properties of experimental preparation on the basis of
Vibrio cholerae O-antigen, O1 and O139 serogroups on the mouse model: determination of toxicity, analysis of morphological changes in organs, and flow-cytometric monitoring of cell cycle of leukocytes, splenocytes and bone marrow cells. It is demonstrated that O-antigen 100 µg dose per mouse does not initiate either significant changes in parenchymatous organs, or imbalance of apoptosis and proliferation of immune-competent cells. Comprehensive assessment of immunobiological properties of the preparations allows to get an insight about their effect on macroorganism, possibility for further studies and their application as chemical vaccine components.

Presented are the results of gene-typing of CCHF virus detected in clinical samples from CHF patients from the Stavropol, Rostov and Astrakhan Regions in 2011. For 28 samples determined are nucleotide sequences of the fragments 115–652 (S segment) and fragments 984–1469 (M segment). Philogenetic analysis of these nucleotide sequences demonstrated that typical strains circulated in 2011 in the regions under surveillance, importation of the new genetic variants of the virus did not take place. CCHF virus variant affiliated to the subgroup “Astrakhan-2” was detected in the clinical samples for the first time and characterized for its genome S- and M-segment fragments.

Laser Doppler flowmetry method (LDF) was used to examine children (43 patients with enteroviral infection, 34 patients with Astrakhan rickettsial fever, and 40 healthy controls). In addition, in view of LDF survey data, carried out was thermal assay and amplitude-frequency analysis of peripheral microcirculation predictors. Detected were incipient changes in microvasculature relative to microangiopathy.

Presented is the information on technical and medical trials of new generation preparations for gene diagnostics of Dengue fever and cholera, based on the multiple factor analysis. Application of these preparations makes it possible not only to detect pathogen but also to carry out its expedited identification in accordance with epidemiological significance and taxonomic status.

Studied is the diagnostic efficiency (specific activity, sensitivity, specificity, reproducibility) of anthrax vegetative fluorescent immunoglobulins. Based on the data, received in medical trials, this preparation is recommended for registration as a product for medical application in the Russian Federation.