The first cases of the disease caused by pandemic (H1N1) 2009 influenza virus in the territory of the Russian Federation were registered at the end of May, 2009. 3 strains of pandemic (H1N1)2009 influenza virus were isolated from patients. Properties of the strains isolated in the territory of Russia were studied in comparison with those of two reference strains A/California/04/2009(H1N1) and A/California/07/2009(H1N1)v. Analysis of primary gene sequence and examination of biological properties of the strains isolated in the territory of Russia suggested their close relationship with A/California/04/2009(H1N1)v and A/California/07/2009(H1N1)v strains.

A unique, many-functional complex of mobile laboratories for SAET on the basis of cross-country chassis was created for the first time. The complex is designed for work in the areas of ES in the sphere of sanitary and epidemiologic welfare of the population. This unparalleled project was carried out in the course of step-by-step modernization of the specialized anti-epidemic teams (SAET) of the Rospotrebnadzor anti-plague institutes in the light of implementation of the conception of SAET modernization and main principles of SAET functioning. Presented are structural and functional characteristics of the complex of SAET mobile laboratories on the basis of cross-country chassis, and engineering and technical means used. Substantiated are relevant levels of biological safety provision for every laboratory. Qualitative advantages of the laboratories on the basis of cross-country chassis are marked, and algorithm of provision of constant readiness of SAET mobile complex is presented.

Kyzylkum autonomous plague focus, the large part of which is situated on the territory of the Republic of Uzbekistan, has always been distinguished as regards periodic development of acute extended epizootics and occurrence of epidemic complications. Epidemic survey includes the wide reconnaissance with visual evaluation of epizootiological factors and material collection out of fresh pellets for serological analysis. In case of discovery of carriers carcasses, suspected as plague infected, the collection of samples into spiritus in view of their further PCR analysis is carried out.

Monthly distribution of epidemic typhus fever morbidity during epidemics arisen since the end of XIX up to the middle of XX century has been analyzed. The cumulative coefficient considering a set of factors which effect the epidemic typhus fever morbidity is substantiated. This coefficient can be used to forecast the scale of this infection outbreaks in case of its introduction from endemic regions or recurrence of Brill's disease.

Evaluated are available materials, having absorbability, in view of using them as adsorbents for transportation of pathogenic biological agents. Worked out is the way of their disinfection

Analysis of many-years brucellosis morbidity in Stavropol region in 2001-2007 showed epidemiological situation to be tense, thus the deterioration of epizootiological and epidemiological conditions as regards brucellosis could be expected. Epidemiological zoning of Stavropol region according to the rate of risk of population infectioning with brucellosis allowed to discriminate territories with different degree of epidemiological danger. These data could be used during next 2-3 years for evaluation of brucellosis epidemiological situation while carrying out epidemiological analysis.

The detailed description of imported cholera cases in Beloretshk, the Republic of Bashkortostan, in 2008 is presented. The cases were associated with pilgrimage in India. The similar case of cholera importation was registered in Beloretsk in 2004. Special attention should be paid to the persons returned from religious trips to India as their long stay in cholera endemic country increases the risk of infection. Should there appear gastrointestinal diseases in persons returned from cholera-endemic countries it is advisable to carry out their examination on cholera using bacteriological and serological methods.

Presented are the field trial results of mobile laboratory in the forest-steppe, steppe and semi-desert regions in autumn and winter periods.

Analysis of the population structure of 41 Vibrio cholerae biovar eltor natural strains permitted to detect 12 strains forming typical transparent and atypical opaque colonies. Among the later were identified 3 strains which morphology variability correlated with altered production of exopolysaccharide, cholera toxin, hemolysine, soluble hemagglutinin/protease and altered mobility. One of the external signals causing coordinating switching of production of the indicated pathogenicity and persistence factors is alkaline (pH 9,0) reaction of the medium.

The characteristics of tick-borne borreliosis agents and borrelia reservoir hosts and vectors are presented in the article. Considered is participation of ticks' saliva components and superficial spirochete antigens in the mechanisms of mammals infectioning with borrelia. Immunopathogenesis of tick-borne borreliosis is described. The available methods of diagnostics and vaccine prophylaxis of tick-borne borreliosis are evaluated.

Evaluated were alterations in thymus, regional and remote lymphatic glands, and spleen of biomodels as well as neuroendocrinal cells reaction in these organs in simulating anti-tularemia vaccinal process. Received are quantitative characteristics of neuroendocrinal environment state of biomodels' lymphoid organs on the 5 day after administration of anti-tularemia vaccine in different doses. Elucidated were certain principles in cells' reaction of APUD-system of biomodel's lymphoid organs and lungs, associated with nature of their alterations. Identified were the prospects of application of the APUD-system cells quantitative characteristics in view of improving and optimizing the evaluation methods as regards safety and efficiency of live vaccines.

Demonstrated was the possibility to use PHOSPHAN microplate technology to examine human sera and detect simultaneously specific IgG antibodies to three arboviruses, including the West Nile virus (WNV), Crimean Congo Hemorrhagic Fever virus (CCHFV), and Tick-borne encephalitis virus (TBEV). Both sensitivity and specificity of microarray immunoassay approach were similar to those of ELISA tests (when serum specimens were investigated separately). Using the criterion of 2-fold or stronger reaction of the examined serum specimen with homologous antigen than with heterologous one, we succeeded in differentiation of group specific anti WNV and anti TBEV IgG antibodies in about 60% of cross reactive sera. From the economical standpoint, the PHOSPAN technology may have advantages as compared with currently used ELISAs due to miniaturization of immunoassay format and ability to multiplex.

Compositional micro-grained immune-magnetic absorbents (IMA) were obtained on the basis of silica - aluminocilicate, modified by polyglucin and sodium secondary alkylsulphate. IMA were shown to possess high adsorption activity due to their developed surface and implanted specific ligand, standard structural features, mechanical reliability, chemical and microbiological stability. The existence of magnetic material provided facilitation and convenience of operations with absorbents in analysis performance. Applying of IMA allowed to release of any mixtures with their adverse effect on reaction during samples preparing by means of rinsing the absorbent with fixed infected agent on it; concentrating the unknown pathogen to the extent possible increased the specificity and sensitivity of express-analysis methods (EIA and PCR), the time spending for analysis reduced substantially (up to 1-3hours).

Has been analyzed the effect of enzyme preparations (trypsin, pepsin, pronase, proteovibrin - enzyme complex isolated from cultural ultrafiltrate of the industrial strain Vibrio cholerae M 41 Ogawa and from non-inactivated autolysate of yeasts) upon the process of autolysis of baker's yeast (Saccharomyses cerevisiae). The selected enzyme preparations being used in small doses render stimulatory effect to autolytic process thus reducing its duration and enabling to obtain autolysate with higher content of amine nitrogen.

5 experimental nutritious bases were prepared out of plant materials (soybeans, soymilk, sugar-beet molasses - sugar syrup), and their physical and chemical parameters and amino-acid composition were studied. Nutrient media developed on experimental basis met the nutritious demands of studied strains of plague, cholera, anthrax microbes and sporogenous saprophytes.

Presented are technologies of production of brucellosis diagnostic bacteriophages developed on the basis of determination of optimal conditions of the phages reproduction. Stability of the finished product was evaluated using Shuhard control charts.

Analyzed were case records of patients with chronic brucellosis for the period of 1994-2009. Presented are the examples from the disease anamnesis of the patients examined in the infectious unit of Krasnoyarsk municipal hospital, with diagnosis of brucellosis established not in time. The reasons of diagnostic mistakes in various forms of brucellosis are considered.